CD4^+CD25^+Foxp3^+T调节细胞与小鼠异基因造血干细胞移植GVHD相关性研究  

The Correlation between Mice CD4^+CD25^+Foxp3^+ T Cells and GVHD

作　　者：杨明珍[1] 吴德沛[1] 陈广华[1] 岑建农[1] 袁燕慧[1] 常惠荣[1] 朱子玲[1] 冯宇锋[1] 

机构地区：[1]苏州大学附属第一医院血液科江苏省血液研究所,江苏苏州215006

出　　处：《中国血液流变学杂志》2007年第3期356-359,369,共5页Chinese Journal of Hemorheology

基　　金：江苏省卫生厅"135工程"重点学科开放课题基金(K0614);苏州大学优秀博士论文选题立项基金资助

摘　　要：目的探讨CD4+CD25+foxp3+调节性T细胞与小鼠GVHD发生的相关性。方法取8～10周龄的SPF级CB6F1小鼠(H-2b/d,♀)30只,随机分为正常对照组、同基因移植组和GVHD组,每组10只,正常对照组不接受照射和移植,同基因移植组小鼠照射后接受CB6F1小鼠骨髓细胞(1×107)和脾细胞(3×107),GVHD组小鼠照射后接受C57BL/6(H-2b,♂)小鼠骨髓细胞(1×107)和脾细胞(3×107)。在发生GVHD的时间点检测3组小鼠脾细胞CD4+CD25+T细胞和foxp3 mRNA的表达。结果正常对照组、同基因移植组和GVHD组小鼠脾细胞中CD4+CD25+T细胞的比例分别为(8.47±1.03)%、(15.40±0.80)%和(24.03±0.85)%,均有显著性差异(P<0.01)。GVHD组小鼠脾细胞foxp3 mRNA的表达水平明显低于正常对照组和同基因移植组,有显著性差异(P<0.001)。结论CD4+CD25+foxp3+调节性T细胞与小鼠GVHD发生呈负相关,发生GVHD时小鼠脾细胞中CD4+CD25+foxp3+调节性T细胞明显减低。Objective To probe the correlation between mice CD4^＋CD25^＋Foxp3^＋ T cells and GVHD of mice allogeneic haemopoietic stem cell transplantation.Methods CB6F1 mice（H-2b/d,♀）were randomized to 3 groups between 8～10 weeks of age,weight was（20± 2）g,control group mice were not given X-ray and transplantation,autologous haemopoietic stem cell transplantation group mice received CB6F1 mice bone marrow cells（1× 107） and spleen cells（3×10^7）after X-ray irradiation,GVHD group mice were given C57BL/6（H-2b,♂）mice bone marrow cells（1 × 107） and spleen cells（3 × 107）after X-ray irradiation.At the time of GVHD happened,3 groups mice spleen cells were prepared,CD4^＋CD25^＋ T cells were detected with FCM（CD4-pcy5,CD25-FITC）,the relative levels of foxp3 mRNA were determined by real-time quantitative RT-PCR in total splenocytes.Results The CD4^＋CD25^＋T cell of mice splenocytes in control group,auto-HSCT group and GVHD group was（8.47 ± 1.03）%,（15.40 ± 0.80） % and（24.03±0.85）%,respectively（P〈0.01）,real-time quantitative RT-PCR showed that the levels of foxp3 mRNA of GVHD group mice splenocytes was lower than control group and auto-HSCT group（P〈0.001）.Conclusion There was a negative correlation between CD4^＋CD25^＋Foxp3^＋T cells and GVHD,CD4^＋CD25^＋foxp3＋T cells were lower when GVHD happened.

关 键 词：造血干细胞移植 移植物抗宿主病 T细胞 CD4^+CD25^+ FOXP3 

分 类 号：R457.7[医药卫生—治疗学]