C/EBP β在实验性矽肺大鼠肺中表达的研究  

Study on the Expression of C/EBP β in Lung of Silica Exposed Rats

作　　者：董磊[1] 朱俊[1] 李春红[1] 吴强[2] 胡天锡[3] 沙泉[1] 

机构地区：[1]安徽医科大学 过敏与免疫研究中心 免疫学教研室,合肥230032 [2]安徽医科大学 病理学教研室,合肥230032 [3]上海市疾病预防控制中心,上海200336

出　　处：《环境与职业医学》2007年第5期490-493,497,共5页Journal of Environmental and Occupational Medicine

基　　金：安徽省优秀青年科技基金(编号:04043053);教育部留学回国人员科研启动资金(编号:2005383);安徽省高校省级自然科学研究重点项目(编号:KJ2007A023)

摘　　要：[目的]探讨转录因子CCAAT-增强子结合蛋白(CCAAT-Enhancer-Binding Protein-beta,C/EBPβ)在矽肺建模大鼠中的表达及其意义。[方法]建立矽肺大鼠模型,运用免疫组织化学方法检测C/EBPβ在30例染尘后不同阶段矽肺大鼠和10例正常大鼠肺组织中的表达,并对C/EBPβ阳性细胞的类型、分布进行分析。[结果]C/EBPβ主要表达于肺脏的上皮细胞及炎性细胞中。染尘后1～2周明显可见C/EBPβ的核移位,C/EBPβ在矽肺大鼠肺组织中表达较正常肺脏组织明显增强,且在染尘后36～64d的大鼠中其表达程度比在1～29d的大鼠中明显减弱,差异有显著性。[结论] C/EBPβ在矽肺大鼠肺脏组织内的多种炎性细胞中明显表达,能客观反映免疫或炎症早期反应的程度,提示C/EBPβ在矽结节产生和进展中可能发挥重要启动作用,其在早期矽肺大鼠肺脏组织上皮细胞中强阳性表达,提示上皮组织可能也是第一道抵御矽肺形成的防线。[ Objective ] To investigate the expression and significance of transcription factor CCAAT-Enhancer-Binding Protein-beta （ C/EBP 13 ） in the development of rat silicosis models. [ Methods ] The experimental silicosis model in rats was established by intratracheal instillation of silica. The expression of C/EBP 13 was determined in thirty lung tissue samples of silica exposed rats at different stage of silicosis while lung tissue samples from ten normal rats were measured as control by immunohistochemistry method. The C/EBP β positive cell type and distribution were analyzed. [ Results ] C/EBP β-positive cells were localized in epithelium and inflammatory cells in lung tissue. C/EBP β nuclear translocation was observed clearly in the 1 - 15 days silicotic tissue. There was a significant over-expression of C/EBP β in lung tissue of silica exposed rats as compared with that in the normal ones. The expression of C/EBP β in the groups of 36 - 64 days was significantly lower than that in the groups of 1 - 29 days after silica instillation. [ Conclusion ] The over-expression of C/EBP β in a variety of inflammatory cells can reflect the extent of immune response or early phase of inflammation objectively. Our results suggest that C/EBP β may play an important initial role in the development of silicotic nodule. The strong expression of C/EBP β in airway epithelium in the early stage of silicosis may also suggest that epithelium is the primary line of defense to resist the formation of silicosis.

关 键 词：CCAAT-增强子结合蛋白-β 矽肺 免疫组织化学 炎症 

分 类 号：R114[医药卫生—卫生毒理学]