锰的遗传毒性及锌对其毒性的拮抗作用  

The Genetic Toxicity of Manganese and Its Antagonism of Zinc

作　　者：樊卫萍[1] 袁淑芳[1] 吉顺福[1] 

机构地区：[1]绵阳师范学院 生命科学与工程系,四川绵阳621000

出　　处：《环境与职业医学》2007年第5期516-518,共3页Journal of Environmental and Occupational Medicine

摘　　要：[目的]研究锰的遗传毒性及锌对其毒性的拮抗作用。[方法]昆明种小鼠分为7组，对照组、染锰组（分3个剂量给小鼠腹腔注射氯化锰）、干预组（注射相应3个剂量的氯化锰并在饮水中添加硫酸锌），6周后检测小鼠胸骨骨髓细胞微核情况，用彗星试验检测其外周血淋巴细胞DNA损伤。[结果]随染锰剂量的增加小鼠骨髓细胞微核率随之上升，中、高染锰组显著高于对照组（矿值分别为63．32，222．82，P〈0．01），淋巴细胞DNA损伤程度逐渐加重，低、中、高染锰组均高于对照组（矿值分别为934．11，3357．21，6277、45，P〈0．01）；锌干预后，加锌染锰组微核率均低于相应不加锌染锰各剂量组，低锰组细胞微核率接近于对照，而加锌中、高锰组与相应不加锌染锰各剂量组比较差异均有统计学意义（矿值分别为2．33，158．27，P〈0．01）。加锌染锰组DNA损伤均低于相应不加锌染锰各剂量组，差异均有统计学意义（矿值分别为83．15，81．66，241．76，P〈0.01）。[结论]过量锰可导致小鼠淋巴细胞DNA损伤，具有遗传毒性，锌对其毒性有一定拮抗作用。[ Objective ] To study the genetic toxicity of manganese and the antagonistic action of zinc in mice. [ Methods ] Kunming mice were randomly divided into seven groups, namely： 1 control group, 3 manganese intoxicated groups in 3 different concentrations of manganese chloride intraperitoneally injected, and other 3 groups with the same treatment as the former but zinc was also fed through drinking water. Six weeks later, the frequencies of micronucleate of polychromatic erythrocytes（ PCE ）in the bone marrow were measured with micronucleus test. The rates of DNA migration were measured with comet assay. [ Results ] ① The frequency of PCE in the bone marrow increased significantly with the increased concentration of manganese, and was higher in medium and high dose groups than in the control group（Х^2 = 63.32,222.82, P 〈 0.01 ）. ②The rates of DNA migration increased significantly with the increased concentration of manganese, and were greater than that in the control group （ Х^2 = 934.11, 3 357.21, 6 277.45, P 〈 0.01 ）. With zinc added, the frequencies of micronucleate in the low dose group decreased to the similar level in control group, and also decreased in medium and high dose groups although still higher than that in the control group, but had significant differences compared with the groups of similar exposure to manganese without zinc fed. The rates of DNA migration in different concentrations of Mn exposure with zinc added were lower than that in the relevant groups without zinc added （Х^2 = 83.15, 81.66,241.76, P 〈 0.01 ）. [ Conclusion ] Manganese has genetic toxicity. However, those effects can be antagonized by zinc.

关 键 词：锰 锌 DNA损伤 

分 类 号：R994.6[医药卫生—毒理学]