良性胆管狭窄组织中CTGF表达的意义  

Expression of CTGF in benign biliary stricture and its significance

作　　者：耿智敏[1] 张晓雪[1] 王林[1] 陶杰[1] 

机构地区：[1]西安交通大学医学院第一附属医院肝胆外科,陕西西安710061

出　　处：《第四军医大学学报》2007年第21期1951-1953,共3页Journal of the Fourth Military Medical University

基　　金：陕西省科技攻关项目(2002K10-G8)

摘　　要：目的:研究良性狭窄胆管组织中CTGF表达的作用.方法:良性狭窄胆管组织23例,正常胆管组织6例,采用免疫组织化学SABC法和原位杂交法检测CTGF和CTGF mRNA的表达.结果:CTGF,CTGF mRNA在良性狭窄胆管组织中主要表达于成纤维细胞胞质内,阳性率分别为82.6%(19/23)和65.2%(15/23);正常胆管组织中少量表达于管壁纤维细胞,阳性率分别为50.0%(3/6)和16.7%(1/6),组间有统计学差异(P<0.05).结论:良性胆管狭窄组织中CTGF呈高表达,是导致胆管良性狭窄的重要因素.AIM： To explore the role of CTGF in benign biliary stricture. METHODS： Paraffin embedded materials from 23 cases of benign biliary stricture were analyzed for CTGF protein expression by immunohistochemical strept-avidin-biotin complex （SABC） method and CTGF mRNA expression by in situ hybri- dization. Meanwhile, 6 normal cases served as controls. RESULTS： CTGF protein and mRNA were expressed mainly in the cytoplasm of fibroblasts in the tissue of benign biliary stricture, and the positive rates were 82.6% （ 19/23 ） and 65.2% （ 15/ 23 ） respectively. Meanwhile, they were expressed slightly in fibrocytes in the normal bile duct, and the positive rates were 50.0% （3/6） and 16.7% （1/6） respectively. There was a sig- nificant difference between the 2 groups （P 〈 0.05）. CONCLU- SION： The high expression of CTGF plays an important role in the formation of benign biliary stricture.

关 键 词：胆管狭窄 结缔组织生长因子 免疫组化 原位杂交 

分 类 号：R657.46[医药卫生—外科学]