银杏叶提取物对哮喘小鼠气道重塑的影响及机制  

Effect of Ginkgo biloba extract on airway remodeling in asthmatic mice and its mechanism

作　　者：李艳[1] 戚好文[1] 张力江[1] 陈旭昕[1] 王洋[1] 宋立强[1] 

机构地区：[1]第四军医大学西京医院呼吸内科,陕西西安710033

出　　处：《第四军医大学学报》2007年第21期1954-1956,共3页Journal of the Fourth Military Medical University

摘　　要：目的:探讨哮喘小鼠气道重塑与基质金属蛋白酶-9(MMP-9)的关系以及银杏叶的干预作用.方法:建立哮喘小鼠气道重塑模型,40只BALB/c小鼠按随机数字法分为4组:对照组,哮喘组,银杏叶干预组,地塞米松干预组.以鸡卵清蛋白(OVA)致敏并激发制备小鼠慢性哮喘模型.对肺组织切片行苏木精-伊红染色观察气道重塑情况.RT-PCR检测各组小鼠肺组织MMP-9 mRNA表达.结果:哮喘组动物出现管壁增厚、平滑肌增生、黏液分泌增加等气道重塑的特征性改变,MMP-9 mRNA水平增高.地塞米松干预组和银杏叶干预组与哮喘组比较,炎症反应轻微,平滑肌增生、粘液分泌不明显,MMP-9 mRNA水平表达降低,与对照组比较差异有统计学意义.结论:长期吸入变应原可导致气道重塑,MMP-9在气道重塑中发挥了作用,银杏叶的干预作用说明在血小板活化因子(PAF)和MMP-9之间可能存在某种联系.AIM： To explore the relationship between airway remodeling and expression of matrix metalloproteinase-9 （ MMP- 9） and the effect of gingko biloba extract in asthmatic mice. METHODS： Forty BALB/c mice were randomly divided into four groops： control groop, asthma groop, gingko biloba extract treatment groop, dexamethasone （DXM） treatment groop. Mice were sensitized and challenged with ovalbumin to establish the asthmatic model. The paraffin embedded sections of lung tissue underwent HE staining and the lung pathological changes were observed. Transcriptional levels of MMP-9 mRNA extracted in lung tissues were assessed by RT-PCR. RESULTS： After repeat- ed allergen challenge, obvious infiltration of inflammatory cells and proliferation of goblet cells and smoothmuscle were demonstra- ted in mouse bronchi. Expression levels of MMP-9 in the epithe- lial cells of bronchi were significantly higher in asthmatic animals than those in control animals. Compared with asthmatic group, there was mild inflammation reaction, decreased in collagen depo- sition and expression of MMP-9 in gingko biloba extract treatment groop and DXM treatment groop, and they were not significantly higher than those in control animals. CONCLUSION： Repeated exposure of allergen induces airway inflammation and remodeling. MMP-9 plays an important role in airway remodeling. Gingko biloba extract intervention demonstrates that PAF has some con- nection with MMP-9.

关 键 词：哮喘 MMP-9 气道重塑 银杏叶 

分 类 号：R965.2[医药卫生—药理学]