血红素类蛋白质—纳米氧化铝—金胶自组装体系的直接电化学与电催化  

Direct electrochemistry and electrocatalysis of heme proteins immobilized on nano-alumina-gold nanoparticles assembly system

作　　者：秦玉华[1] 秦玉春[1] 关晓辉[1] 周立恒[1] 王胜天[2] 

机构地区：[1]东北电力大学化学工程学院,吉林吉林132012 [2]东北师范大学化学学院,吉林长春130024

出　　处：《兰州大学学报（自然科学版）》2007年第5期54-59,共6页Journal of Lanzhou University(Natural Sciences)

基　　金：Supported by National 863 Plan Foundation of China(2002AA233051).

摘　　要：三种血红素类蛋白质——细胞色素c、肌红蛋白、血红蛋白,被固定在纳米氧化铝—金胶自组装体系修饰玻碳电极表面,紫外光谱实验结果表明固定在纳米氧化铝—金胶表面的蛋白质保持其原始的二级结构不变.用电化学阻抗光谱和循环伏安技术表征了界面的组装过程及其电化学性质,结果表明纳米氧化铝—金胶模板不仅为蛋白质固定提供了良好的环境,而且加快了蛋白质分子与电极之间的电子转移.讨论了扫描速度对细胞色素c电化学行为的影响及其对过氧化氢的电催化还原等性质.Three heme proteins, cytochrome c, myoglobin and hemoglobin were adsorbed on the nanoporous alumina （AAO） templates-gold nanoparticles （GNs） modified glassy carbon electrodes （GCE）. CV-vis spectroscopy indicated that the proteins immobilized on AAO-GNs films retained near-native secondary structures. The fabricated procedures and electrochemical behaviors of proteins on such an interface were characterized with electrochemical impedance spectra and cyclic voltammetric techniques. It was demonstrated that the AAO-GNs films not only could offer a friendly environment to immobilize the protein molecules, but also enhance the electron transfer ability between protein molecules and the underlying electrodes. The effects of scan rate on the electrochemical behaviors of cytochrome c and the electrocatalytic response to the reduction of H2O2 were discussed in detail.

关 键 词：细胞色素C 肌红蛋白 血红蛋白 纳米氧化铝 金胶 直接电化学 

分 类 号：O657[理学—分析化学]