黄芪多糖对细菌脂多糖诱导仔猪腹腔巨噬细胞分泌TNF-α、NO及IL-1的影响  

Effect of astragalus polysaccharides on IL-1,NO and TNF-α secreted by lipopolysaccharid induced normal piglets peritoneal macrophages

作　　者：欧德渊[1] 高铭宇[2] 田兴贵[1] 杨东梅[1] 付学志[1] 

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]中国农业大学科学技术处

出　　处：《贵州畜牧兽医》2007年第5期1-3,共3页Guizhou Journal of Animal Husbandry & Veterinary Medicine

基　　金：贵阳市科技发展基金课题(2005筑科农字第4-10号);贵州大学人才基金资助

摘　　要：目的:研究黄芪多糖(APS)对细菌脂多糖(LPS)诱导仔猪腹腔巨噬细胞肿瘤坏死因子(TNF-α)基因表达、白细胞介素-1(IL-1)及一氧化氮(NO)的影响。方法:取仔猪腹腔巨噬细胞进行体外培养,用APS和LPS处理,分完全培养液组、APS组、APS+LPS组和LPS组四个处理。采用实时定量PCR方法检测TNF-α基因表达,TNF-α和IL-1蛋白含量采用放免法,NO含量采用Griess法测定。结果:完全培养液组、APS组、APS+LPS组TNF-α mRNA的表达显著或极显著低于LPS组(P<0.05或P<0.01)。完全培养液组、APS组、APS+LPS组上清液中TNF-α、IL-1和NO的含量显著或极显著低于LPS组(P<0.05或P<0.01)。结论:APS抑制仔猪巨噬细胞TNF-α表达及分泌炎性因子,这可能是APS维持仔猪健康的重要机制之一。To study effect of astragalus polysaccharides （APS）on IL - 1, NO and TNF -α secreted by piglets peritoneal macrophages induced by lipopolysaccharide （LPS）. Methods： piglets peritoneal macrophages were cocuhured with APS （100 mg/L）and/or LPS （50 mg/L）in vitro. The expression of TNF- α mRNA was measured by real time, production of NO was determined by Griess assay, activity of interleukin- 1 （IL- 1 ）was assayed by radioimmunoassay. Resuhs ：APS could markedly（P 〈0. 05 ）inhibit production of TNF -ct,NO and IL- 1 of piglets peritoneal macrophages induced by LPS. Conclusion：The inhibiting direct secretion of TNF- α, NO and IL - 1 produced by peritoneal macrophages might be one of the mechanisms of anti - inflammatory effect of APS in piglets.

关 键 词：黄芪多糖 肿瘤坏死因子-Α 白细胞介素-1 一氧化氮 仔猪 

分 类 号：Q691.5[生物学—生物物理学]