齿裂菌属一些种内及近似种间遗传多样性的RAPD分子标记  

RAPD markers of some intraspecific and similar interspecific strains of Coccomyces

作　　者：蒋美俊[1] 王士娟[1] 李占东[2] 张韬[1] 林英任[2] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]安徽农业大学林学与园林学院,合肥230036

出　　处：《安徽农业大学学报》2007年第4期520-523,共4页Journal of Anhui Agricultural University

基　　金：国家自然科学基金项目(30370011;30470014)资助

摘　　要：用筛选出的重复性好、条带清晰的14个随机引物分别对采自安徽黄山和天堂寨的12个齿裂菌属(Coccomyces)菌株进行了RAPD分析,其结果与表型特征表现出良好的一致性,从而表明RAPD技术可以较好地应用于齿裂菌属种内和近似种间亲缘关系的分析。通过RAPD分析的结果与表型性状的比较和分析,明确了部分表型性状在齿裂菌属近似种区分方面的重要性。Twelve Coccomyces strains from Huangshan and Tiantangzhai were characterized by RAPD markers with 14 random primers. The primers used in the reaction were selected from 100 random primers and their gel elec- trophoresis diagram can be amplified repeatedly and clearly. The results of RAPD analysis were consisted with their phenotypic characters very well, which indicated that the RAPD technology could be used to analyze the relation- ships of some intraspecies and similar interspecies of Coccomyces efficiently. Comparing to the results of RAPD anal- ysis and their phenotypic characters, the importance of partial phenotypic characters were affirmed in discriminating of similar species of Coccomyces.

关 键 词：齿裂茵属 RAPD标记 遗传多样性 

分 类 号：Q949.32[生物学—植物学]