Characterization of SNAP-25 gene from marine teleostean, Lateolabrax japonicus  

作　　者：陈魁 黄晓航 柴迎梅 Herbert Y. Gaisano 

机构地区：[1]Institute of Oceanology Chinese Academy of Sciences [2]The First Institute of Oceanography, State Oceanic Administration [3]Department of Physiology, University of Toronto, Toronto, M5S 1A8, Canada

出　　处：《Chinese Journal of Oceanology and Limnology》2007年第4期378-385,共8页中国海洋湖沼学报（英文版）

基　　金：the NSFC (No.40476060);Hi-Tech Research and Development Program of China (No. 2002AA629120)

摘　　要：The t-SNARE protein SNAP-25 (synaptosome-associated protein of 25 kDa) plays an essential role in regulating fusion between the vesicle and plasma membranes during exocytosis. To clone and characterize SNAP-25 gene, the first step in the functional study of SNARE proteins in marine teleostean, was to obtain the cDNA of sea perch SNAP-25 (SPsn25) by RT-PCR and RACE-PCR amplification of a Japanese sea perch. The full-length cDNA of 831bp contains a CDS of 615 bp, coding 204 amino acid residues, and a 5′UTR of 219bp. Bioinformatic analysis revealed that SPsn25 corresponds with SNAP-25a isoform and shares 91.1% identity with SNAP-25a of a goldfish and a zebrafish. The SPsn25 expression in both mRNA and protein levels in the Japanese sea perch had been identified through semi-quantitative RT-PCR and Western Blot assay. Together, these data again confirmed the nerve tissue specificity of the fish SNAP-25 gene expression.The t-SNARE protein SNAP-25 （synaptosome-associated protein of 25 kDa） plays an essential role in regulating fusion between the vesicle and plasma membranes during exocytosis. To clone and characterize SNAP-25 gene, the first step in the functional study of SNARE proteins in marine teleostean, was to obtain the cDNA of sea perch SNAP-25 （SPsn25） by RT-PCR and RACE-PCR amplification of a Japanese sea perch. The full-length cDNA of 831 bp contains a CDS of 615 bp, coding 204 amino acid residues, and a 5＇UTR of 219bp. Bioinformatic analysis revealed that SPsn25 corresponds with SNAP-25a isoform and shares 91.1% identity with SNAP-25a of a goldfish and a zebrafish. The SPsn25 expression in both mRNA and protein levels in the Japanese sea perch had been identified through semi-quantitative RT-PCR and Western Blot assay. Together, these data again confirmed the nerve tissue specificity of the fish SNAP-25 gene expression.

关 键 词：Lateolabrax japonicus SNARE SNAP-25 

分 类 号：Q75[生物学—分子生物学]