Identification and characterization of pathogen to bacterial septicaemia in cultured turbot, Scophthalmus maximus  

作　　者：秦国民 张晓君 陈翠珍 房海 战文斌 

机构地区：[1]Key Laboratory of Oceanic Biotechnology of Jiangsu, Huaihai Institute of Technology [2]Department of Animal Science, Hebei Normal University of Science and Technology [3]The MOE Key Laboratory of Mariculture, Ocean University of China

出　　处：《Chinese Journal of Oceanology and Limnology》2007年第4期403-410,共8页中国海洋湖沼学报（英文版）

基　　金：Major State Basic Research Development Program (No. G1999012002);Science Technology Department Project of Hebei Province (No. 302431)

摘　　要：Bacteria-infected turbots Scophthalmus maximus with septicaemia were examined between 2001 and 2004 in aspects of the conditions of disease occurrence, clinical syndromes and pathological changes. The phenotypic information of pathogenic bacteria was studied, including morphology, physiological and biochemical characteristics, and the mol% G+C of the DNA. In addition, representative strains (S010623-1, LH031120-1) were selected for molecular identification by partial 16S rRNA gene sequencing. The results show that the isolates (LH031120-1 to LH031120-6, HT040308-1 to HT040308-6, HT040620-1 to HT040620-6) from three farms were identified as Edwardsiella tarda. The isolates (S010610-1 to S010610-10, S010623-1 to S010623-20) from one farm were identified as Listonella anguillarum. We conducted studies on the pathogenicity of isolates by artificial infection, and revealed all infected groups in morbidity and mortality. The septicaemia infected turbot showed a syndrome similar to that of the naturally infected fish. Antibiotic sensitivity showed that of 37 antimicrobial agents, E. tarda was sensitive to 27 agents, and L. anguillarum was sensitive to 21 agents.Bacteria-infected turbots Scophthalmus maximus with septicaemia were examined between 2001 and 2004 in aspects of the conditions of disease occurrence, clinical syndromes and pathological changes. The phenotypic information of pathogenic bacteria was studied, including morphology, physiological and biochemical characteristics, and the mol% G＋C of the DNA. In addition, representative strains （S010623-1, LH031120-1） were selected for molecular identification by partial 16S rRNA gene sequencing. The results show that the isolates （LH031120-1 to LH031120-6, HT040308-1 to HT040308-6, HT040620-1 to HT040620-6） from three farms were identified as Edwardsiella tarda. The isolates （S010610-1 to S010610-10, S010623-1 to S010623-20） from one farm were identified as Listonella anguillarum. We conducted studies on the pathogenicity of isolates by artificial infection, and revealed all infected groups in morbidity and mortality. The septicaemia infected turbot showed a syndrome similar to that of the naturally infected fish. Antibiotic sensitivity showed that of 37 antimicrobial agents, E. tarda was sensitive to 27 agents, and L. anguillarum was sensitive to 21 agents.

关 键 词：Turbot （Scophthalmus maximus L.） Edwardsiella tarda Listonella anguillarum CHARACTERIZATION SEPTICAEMIA 

分 类 号：S943[农业科学—水产养殖]