先进的微生物芯片检测系统及其应用  

Novel Detection System of Microbe Chip and Its Application

作　　者：黄国亮[1] 邓橙[1] 徐书宽[1] 朱疆[1] 董中华[1] 杨阳[1] 阳小勇[2] 张亮[2] 

机构地区：[1]清华大学生物科学与技术系 [2]生物芯片北京国家工程研究中心,北京102206

出　　处：《光学学报》2007年第3期499-504,共6页Acta Optica Sinica

基　　金：国家863计划(2006AA020701;2006AA020803);国家973计划(2006CB705700);浙江省科技攻关重点项目(2006C21G3210005);清华大学基础研究基金(052205003);清华-裕元医学科学研究基金(40000510B)资助课题。

摘　　要：发展了一种先进的微生物芯片检测方法,并研制用于芯片检测的新型数字化成像扫描检测系统。采用激光诱导荧光的检测原理设计一种新颖的CCD数字化成像扫描检测系统结构,荧光信号采集端的数值孔径NA=0．72,工作距离3．22 mm,系统检测灵敏度小于每平方微米1个荧光分子。以微生物大肠杆菌和黄单胞菌检测为例,设计基因芯片,并应用所研制的芯片检测系统实现了微生物的正确鉴定,提供了一种高效的食品安全检测整体解决方法。实验结果表明两种微生物的芯片检测实验结果稳定可靠,与国外共焦扫描仪检测的结果完全一致。An advanced microbe chip testing method was put forward, and a new digital imaging scanning system for testing biochips was developed. A novel CCD scanning system with laser induced fluorescence for biochip detection was proposed, with a high numerical aperture NA =0.72 for collecting fluorescence, long working distance 3.22 mm and high sensitivity less than 1 fluorescent molecule/μm^2. Microbe chip was designed and tested by the above novel CCD scanning system, which provided a whole solution scheme for efficient food safty inspection. An example of testing escherichia coli and xanthomonas campestris was shown and proved the stability and creditability of the system. The experimental results obtained by the developed digital imaging scanning system indicated the same correct identification of microbe as the commercial confocal scanner.

关 键 词：医用光学与生物技术 CCD扫描检测系统 荧光 微生物芯片 

分 类 号：O43[机械工程—光学工程]