1-烯丙基氯-3对神经细胞胞浆膜和线粒体膜Ca^(2+)-ATPase、Na^+/K^+-ATPase活性的影响  

THE EFFECTS OF CA-ATPaseANDNa/K-ATPase ATIVITIESIN NERVE CELL INDUCEK BY 3-CHLOROPROPENE-1

作　　者：谢克勤[1] 张磊[1] 高树君[1] 孙克任[1] 

机构地区：[1]山东医科大学毒理学研究室

出　　处：《山东医科大学学报》1997年第2期127-131,共5页Acta Academiae Medicinae Shandong

基　　金：国家自然科学基金

摘　　要：用鸡胚脑神经细胞研究１－烯丙基氯－３对细胞胞浆膜和线粒体膜Ｃａ２＋ＡＴＰａｓｅ、Ｎａ＋／Ｋ＋－ＡＴＰａｓｅ活性的影响。结果表明，胞浆膜Ｃａ２＋－ＰＴＰａｓｅ和Ｎａ／Ｋ＋ＡＴＰａｓｅ活性随毒物剂量增加而明显增高，染毒组与对照组相比差异均有显著性（Ｐ＜０。０１）：线粒体膜Ｃａ２＋－ＡＴＰａｓｅ活性随毒物剂量增加而有下降趋势；Ｎａ＋／Ｋ＋ＡＴＰａｓｅ活性则呈增加趋势。酶组化染色结果显示，Ｃａ２＋－ＡＴＰａｓｅ活性与对照组相比明显增加，黑色沉淀明显增多。提示１－烯丙基氯－３中毒后可引起神经钩胞Ｃａ２＋－ＡＴＰａｓｅ和Ｎａ＋／Ｋ＋－ＡＴＰａｓｅ活性改变，这可能与中毒后细胞胞浆和线粒体内的Ｃａ２＋、Ｎａ＋、Ｋ＋的浓度改变有关。Using chicken embryon whole brain cell , the 3-chloropropene- 1 -induced changes of Ca -ATPase and Na /K -ATPase activities in the cytoplasma and mitochondrion membranes were .tudied . The results showed that the activities of Ca - ATPase and Na+/K -ATPase in the cytoplasm membrane increased sharply with toxic dosages added Compared with the control group , both of the toxic groups had significance of differences (P<0. 01 ) . In the mitochondrial membrane , the activities of Ca -ATPase tended to go down with 3-chloropropene-l increased. However , the activities of Na /K -ATPase were opposite and tended to up. The results of enzymatic tissue chemistry also showed that the activities of Ca -ATPase increased. The black sediment in the toxic groups was much more than that of cotttrol These results can suggest that 3-chloropropene- 1 could result in the activity changes of Ca -ATPase and Na /K -ATPase. The changes might be related to the variations of Ca. Na and K contents in the cytoplasm and mitochondrion of the nerve cell

关 键 词：神经细胞 腺苷三磷酸酶 钙 钠 钾 毒理学 

分 类 号：R995[医药卫生—毒理学] R994.1[医药卫生—药学]