捷安肽素抗真菌作用机理研究  

A Research on Mechanism of Antifungal Polypeptide Jiean-peptide

作　　者：代富英[1] 周金燕[2] 谭红[2] 

机构地区：[1]成都医学院病原生物学教研室,成都610083 [2]中国科学院成都生物研究所,成都610041

出　　处：《成都医学院学报》2006年第2期86-90,共5页Journal of Chengdu Medical College

基　　金：中国科学院知识创新工程重要方向项目(No.KSCXZ-SW-301-05)

摘　　要：目的研究捷安肽素的抗真菌作用机理。方法采用形态学方法和同位素标记法。显微形态观察经捷安肽素处理后的供试真菌的形态学变化。进一步采用14C同位素标记的特异底物"尿苷二磷酸-(14C)-葡萄糖"示踪,研究捷安肽素对真菌(1,3)-β-D-葡聚糖合成酶活性反应的影响。结果显微形态观察表明,经捷安肽素处理后,供试真菌呈现球形膨胀,而且随着捷安肽素作用强度增强,球形膨胀的程度和发生频率也增加,提示捷安肽素的作用位点在真菌细胞壁。同位素标记实验表明,捷安肽素可抑制(1,3)-β-D-葡聚糖合成酶的活性,降低真菌细胞壁合成过程中(1,3)-β-D-葡聚糖的放射性掺入,对疫霉菌和辣椒炭疽病菌(1,3)-β-D-葡聚糖合成酶的抑制率分别达到77.7%和77.5%。结论捷安肽素的抗真菌作用机理为:抑制细胞(1,3)-β-D-葡聚糖合成酶的活性,破坏真菌细胞壁的合成,造成真菌细胞壁组分(1,3)-β-D-葡聚糖的缺损和病原真菌生长抑制。Objective To investigate the antifungal mechanism of Jiean - peptide. Methods The mode of action of Jiean - peptide against 4 phytopathogenic filamentous fungi associated with phytopathy： Phytopluhora sp., Colletotrichum gossypii, Southw Botrytis cinerea and Fusariura oxysporum f. sp. vasinfectura was investigated by the method of morphology and isotope labelling. The （1, 3） - β - D - glucan synthase assay was processed using isotope labelled substrate-urdine diphosphate （^14C） glucose to study the effect of Jiean - peptide on fungal （ 1, 3） - β - D - glucan synthase. Results Micmgraphic observation revealed that the Jiean - peptide made the hyphal expanded and globular. The （ 1, 3） - β- D - glucan synthase assay showed Jiean - peptide could inhibit the activity of （ 1, 3） - β - D - glucan synthase and reduce the radioactivity incorporation of （ 1, 3） - β - D - glucan. The inhibition percentages of Jiean- peptide against Phytopluhora sp. and Colletotrichum gossypii Southw were 77.7 % and 77.5% respectively. Conclusion The antifungal mechanism of Jiean - peptide was the inhibition of enzyme activity of （ 1, 3） - β- D- glucan synthase.

关 键 词：捷安肽素 抗真菌作用机理 (1 3)-β—D-葡聚糖合成酶 

分 类 号：R978.16[医药卫生—药品]