穿琥宁注射液中脱水穿心莲内酯琥珀酸半酯的含量测定  

Determination of the Content of Dehydroandyograpolide Succinate in Potassium Dehydroandrograpolide Succinate Injection by HPLC

作　　者：申颖 申和平 

机构地区：[1]湖南省衡阳市药品检验所,湖南衡阳421001

出　　处：《中国药业》2007年第7期22-23,共2页China Pharmaceuticals

摘　　要：目的建立高效液相色谱法(HPLC法)并测定穿琥宁注射液中脱水穿心莲内酯琥珀酸半酯的含量。方法采用C18柱,以0.05%磷酸二氢钾溶液-甲醇(3∶7)为流动相,检测波长为251nm。结果脱水穿心莲内酯琥珀酸半酯质量浓度在2.01～20.05μg/mL范围内与峰面积呈良好的线性关系,平均加样回收率为98.9%,RSD为0.7%。结论HPLC法简便、快速,测定结果准确可靠。Objective To establish a method for the quantitative analysis of dehydroandrograpolide succinate in potassium dehydroandrograpolide succinate injection by HPLC. Methods Using a column packed with octadecylsilane bonded sillica gel and a mixture of 0. 05% potassium dihydrogen phosphate-methanol （3：7） as the mobile phase. The detection wavelength was 251 nm. Results The linear range of dehydroandrograpolide succinate was 2. 01-20. 05 μg/mL and the mean recovery rate of dehydroandrograpolide succinate was 98.9%, RSD =0.7%. Conclusion The method is simple, rapid, accurate and suitable for the content determination of potassium dehydroandrograpolide succinate injection.

关 键 词：高效液相色谱法 穿琥宁注射液 脱水穿心莲内酯琥珀酸半酯 含量测定 

分 类 号：R927.2[医药卫生—药学] R978.7