牛角瓜的组织培养  

Tissue culture of Calotropis gigantea

作　　者：李克烈[1] 罗联忠[1] 陈伟[1] 徐立[1] 李志英 马千全[1] 黄碧兰[1] 

机构地区：[1]中国热带农业科学院作物品种资源研究所,海南儋州571737

出　　处：《广西农业生物科学》2007年第3期247-249,共3页Journal of Guangxi Agricultural and Biological Science

基　　金：国家科技基础条件平台建设项目(2005DKA21000)

摘　　要：以牛角瓜的叶片为外植体,进行牛角瓜的组织培养。结果表明,培养基MS+2,4-D 2 mg/L利于愈伤组织的诱导;培养基MS+6-BA 0.5 mg/L+NAA 0.1 mg/L有利于芽的分化和增殖;生根壮苗的培养基为1/2 MS+NAA 0.5 mg/L,其生根率100%;试管苗生根培养30 d后移栽,成活率可达85%以上。Explants from leaves of Calotropis gigantea were used to establish culture in vitro and regeneration system . The result showed that MS＋2,4-D 2 mg/L was the best medium for callus, and MS＋ 6-BA 0.5 mg/L＋NAA 0.1 mg/L was the best medium for the induce and multiplication, and 1/2 MS ＋ NAA 0.5 mg/L was the best medium for shoot rooting and the rate of rooting was 100%. The shoots were transplanted after 30 days culture in rooting medium. The survival rate after transplantation is above 85%.

关 键 词：牛角瓜 叶片 组织培养 

分 类 号：S567.239[农业科学—中草药栽培]