健肌宁对实验性重症肌无力大鼠差异蛋白表达的调节作用  

Modulation of Jianjining Recipe on differential protein expression in rats with experimental autoimmune myasthenia gravis

作　　者：刘萍[1] 丁旭峰[1] 章怡祎[1] 乔健[2] 

机构地区：[1]上海中医药大学龙华医院,上海200032 [2]复旦大学华山医院神经病学研究所,上海200040

出　　处：《中西医结合学报》2007年第6期642-646,共5页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金(No.30472204)

摘　　要：目的:观察中药复方健肌宁对实验性自身免疫性重症肌无力(experimental autoimmune myasthenia gravis,EAMG)大鼠脾脏差异蛋白表达的调控作用。方法:制备实验性EAMG大鼠模型,随机分为模型组、中药复方健肌宁组、强肌健力胶囊组和醋酸泼尼松龙组。采用双向电泳-串联质谱法分离和鉴定EAMG大鼠脾脏和正常组间的差异表达蛋白,并观察应用健肌宁、强肌健力胶囊和醋酸泼尼松龙连续干预4周后,各组差异蛋白表达水平的变化。结果:在已鉴定的12个疾病相关蛋白中,健肌宁对于同种异体移植炎性因子-1、过氧化氢酶Ⅰ和肌动蛋白相关蛋白2/3复合体亚单位5的蛋白表达水平有显著调控作用(P<0.01),这些蛋白多与免疫反应和细胞运动密切相关。结论:EAMG大鼠与正常大鼠间有差异蛋白表达,具有补益脾肾作用的中药复方健肌宁可以调节部分差异蛋白的表达水平。Objective： To medicine for study the modulation of Jianjining Recipe （JJNR）, a traditional Chinese compound herbal nvigorating spleen and kidney on differential protein expression in spleen of rats with experimental autoimmune myasthenia gravis （EAMG）. Metheds： EAMG rats were randomly divided into four groups： untreated group, JJNR-treated group, Qiangji Jianli capsule （QJJLC, a traditional Chinese compound herbal medicine ）-treated group and prednisolone acetate （PA）-treated group. After therapeutic intervention with the above drugs for four consecutive weeks, the level of differential protein expression was analyzed by two-dimensional electrophoresis and matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Results： Twelve differential proteins were identified by comparing EAMG rsts and normal rats. The levels of allograft inflammatory factor-1, peroxiredoxin Ⅰ and actin-related protein 2/3 complex subunit 5 were significantly regulated by JJNR （P〈0. 01）. These proteins were closely associated with immune response and cell movement. Conclusion： The results suggest that there are differential protein expressions between EAMG rats and normal rats. Furthermore, as a Chinese medicine prescription with effect of invigorating spleen and kidney, JJNR can effectively regulate the levels of some EAMG-related protein expression.

关 键 词：重症肌无力 实验性自身免疫性 脾 电泳 凝胶 双向 

分 类 号：R285.5[医药卫生—中药学]