祛瘀生肌法对大鼠糖尿病创面新生肉芽组织中Bax和Bcl-2的影响  

Effects of resolving stagnation and promoting granulation therapy on expressions of Bax and Bcl-2 in granulation tissue of diabetic rats during wound healing

作　　者：李福伦[1] 李斌[1] 王振宜[1] 范斌[1] 徐文彬[1] 徐蓉[1] 

机构地区：[1]上海中医药大学岳阳中西医结合医院皮肤科,上海200437

出　　处：《中西医结合学报》2007年第6期661-664,共4页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金(No.39900195);上海市重点学科建设资助项目(No.T0304)

摘　　要：目的:探讨祛瘀生肌法促进糖尿病皮肤溃疡大鼠模型创面愈合的辨证用药规律。方法:72只SD大鼠尾静脉注射四氧嘧啶造成糖尿病模型后,剪去背部全层皮肤造成糖尿病皮肤溃疡,将其随机分成生肌化瘀方1(生肌方:化瘀方=1:2)组、生肌化瘀方2(生肌方:化瘀方=1:1)组和生理盐水对照组。分别干创伤后第3天、第7天和第11天取创面新生肉芽组织。采用免疫组织化学法检测新生肉芽组织中Bax和Bcl-2蛋白表达。结果:生肌化瘀方1组创面愈合时间较生肌化瘀方2组和生理盐水组短(P<0.05)。创面修复第3天,各组均未检测到Bax和Bcl-2表达。创面修复第7天和第11天,与生理盐水组和生肌化瘀方2组比较,生肌化瘀方1组新生肉芽组织中Bax和Bcl-2呈阳性表达(P<0.05)。结论:祛瘀生肌中药可调节糖尿病大鼠模型创面新生肉芽组织中Bax和Bcl-2蛋白表达,是祛瘀生肌法促进创面愈合的原因之一。重用化瘀药物有利于创面愈合。Objective： To study the effects of resolving stagnation and promoting granulation therapy on expressions of Bax and Bcl-2 in granulation tissue of diabetic rats during wound healing. Methods： Seventy-two male SD diabetic rats with full-thickness skin lesion were randomly divided into 3 groups： SJHYR 1-treated group, SJHYR 2-treated group and normal saline （NS） control group. SJHYR 1 was prepared with Shengji Recipe （SJR, a compound traditional Chinese herbal medicine for promoting granulation） and Huayu Recipe （HYR, a compound traditional Chinese herbal medicine for resolving stagnation） at a ratio of 1 ： 2, while SJHYR 2 was prepared with SJR and HYR at a ratio of 1 ： 1. Immunohistochemical method was used to assess Bax and Bcl-2 protein levels in granulation tissue. Results： SJHYR 1 could accelerate wound healing as compared with SJHYR 2 and NS （P〈0.05）. On the third day in experiment, Bax and Bcl-2 proteins were not found in any groups, but on the seventh and eleventh day in experiment, Bax and Bcl-2 proteins in SJHYR 1-treated group were much higher than those in the other two groups （P〈0.05）. Conclusion： SJR and HYR in different ratios may all have a role in regulating Bax and Bcl-2 expression in granulation tissue of diabetic rats during wound healing.

关 键 词：糖尿病 溃疡 伤口愈合 BCL-2基因 大鼠 

分 类 号：R275[医药卫生—中医皮科]