血管活性肠肽对大鼠Leydig细胞的免疫调节作用  

Immunoregulation of vasoactive intestinal peptide on rat Leydig cells

作　　者：王伟[1] 席晔斌[1] 陈广洁[1] 郝静[1] 沈天伟[1] 王保国[1] 李伟毅[1] 

机构地区：[1]上海交通大学医学院上海市免疫学研究所,上海200025

出　　处：《细胞与分子免疫学杂志》2007年第8期696-700,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：上海市教委科研基金资助(04BB21);上海市重点学科建设项目资助(T0206)

摘　　要：目的:研究血管活性肠肽(VIP)对大鼠睾丸间质细胞(Leydig细胞)免疫功能的调节。方法:以VIP作用于溶脲脲原体(UU)感染的SD大鼠及大鼠Leydig细胞为研究对象,观察VIP对大鼠Leydig细胞分泌的细胞因子(IL-1、IL-6及TGF-β)和FasL表达的调节,从体外及动物整体水平来探讨VIP对大鼠Leydig细胞免疫功能的调节。结果:在睾丸局部感染时,VIP能调节Leydig细胞IL-1、IL-6、TGF-β及FasL的分泌和表达格局;同时VIP对大鼠睾丸局部免疫豁免的调节和维持也起到一定的作用。结论:VIP能调节大鼠Leydig细胞的免疫功能,并参与调节大鼠睾丸局部的免疫豁免。AIM： To study the regulation of immune function of rat Leydig cells by vasoactive intestinal peptide （VIP）. METHODS： Rat Leydig cells were seperated and infected by UU, with or without the incubation with VlP. The expression of FasL on Leydig cells in different group was analysed by flow cytometry （FCM）. The mRNA expression of IL-1, IL-6, TGF-13 and FasL of Leydig cells in different group was identified by RT-PCR. SD rats were infected by UU with or without the injection of VIP, and the testis tissnes of each group were har vested and observed under transmission electron microscope. RESULTS： When testis was infected by UU, VIP up-regulated the mRNA expression of IL-1, IL-6, and TGF-13, and down-regulated the expression of FasL in vitro. In addition, there was significant difference in testis rnorphous of rats from different group. CONCLUSION： VIP could regulate the expression pattern of IL-1, IL-6, TGF-13 and FasL by Leydig cells, which may contribute to maintain immune privilege of the testis. VIP could regulate rat Leydig cell immune function.

关 键 词：LEYDIG细胞 免疫调节 IL-1 IL- 6 TGF-β FASL 

分 类 号：R329.12[医药卫生—人体解剖和组织胚胎学] R392.23[医药卫生—基础医学]