中国恒河猴(Macaca mulatta)外周血CD4^+CD25^+T淋巴细胞的研究  

Characterization of CD4^+CD25^+T lymphocytes in the peripheral blood of Chinese rhesus macaques(Macaca mulatta)

作　　者：杨贵波[1] 赵辉[1] 邱趁丽[1] 邵一鸣 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心传染病预防控制国家重点实验室,北京100050

出　　处：《细胞与分子免疫学杂志》2007年第8期734-736,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30571750);盖茨基金异体黏膜艾滋疫苗项目(2006年)

摘　　要：目的:研究中国恒河猴外周血中CD4+CD25+T淋巴细胞亚群及其分布频率。方法:利用流式细胞术对50只中国恒河猴外周血CD4+CD25+T淋巴细胞进行了分析。结果:发现所有被检测的恒河猴个体中均存在明显的CD4+CD25+T淋巴细胞亚群;CD4+CD25+T淋巴细胞大约占CD4+T淋巴细胞的9.1%(变化范围为2.6%～18.1%);其中CD4+CD25highT淋巴细胞约占2.5%(0.3%～5.5%)。对不同年龄和性别个体中CD4+CD25+T淋巴细胞频率的初步分析未发现统计学上有年龄或性别差异。结论:中国恒河猴可用于与CD4+CD25+T细胞相关的人类疾病的研究中。AIM： To characterize the CD4^ ＋ CD25 ^＋T lym- phocyte subsets in the peripheral blood of Chinese rhesus macaques. METHODS CD3^＋ , CD4^＋ and CD25^＋ cells in the peripheral blood of 50 Chinese origin rhesus macaques were analyzed by three-color flow cytometry. RESULTS： It was found that all individuals had circulating CD4^＋ CD25 ^＋ T lym- phocytes. In average, 9.1% （ranging from 2.6% to 18.1% ） of CD4^＋ T cells were CD4^＋ CD25^＋ T lymphocytes. CD3 ^＋ CD4^＋ CD25^high cells represented only 2.5% （0.3% to 5.5%） of the CD4^＋ T lymphocytes. No statistically significant differences in the frequency of CD4^＋ CD25^＋ T lymphocytes or their subsets were observed between different age groups or between the male and the female rhesus macaques. CONCLU- SION： The frequency of CD4^＋ CD25^＋ T cells and CD4^＋ CD25^highT cells in the peripheral blood of Chinese rhesus macaques is similar to that in human peripheral blood, indicating that Chinese rhesus macaques may be very useful in bio-medical research on human diseases related to CD4 ^＋ CD25 ^＋ T lymphocytes.

关 键 词：中国恒河猴 调节性T淋巴细胞 CD4^+CD25^+T淋巴细胞 CD4^+CD25^HIGH T淋巴细胞 

分 类 号：R392.11[医药卫生—免疫学]