PTTG、PCNA蛋白在胶质瘤中的表达及其意义  

Expressions and significance of PTTG and PCNA protein in glioma

作　　者：王东[1] 戴如飞[2] 刘宁[3] 李英斌[1] 蔡军[2] 阎超[2] 

机构地区：[1]南京医科大学附属南京第一医院神经外科,江苏南京210006 [2]徐州医学院第二附属医院神经外科,江苏徐州221006 [3]南京医科大学第一附属医院神经外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2007年第9期1013-1015,共3页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省高校自然科学研究计划课题(05KJD320238)

摘　　要：目的:探讨垂体瘤转化基因(pituitary tumor transforming gene,PTTG)、细胞增殖核抗原(proliferating cell nuclear antigen,PCNA)蛋白在胶质瘤中的表达及二者的相互关系。方法:采用免疫组化SP法检测80例胶质瘤中PTTG和PCNA蛋白表达情况。结果:PTTG蛋白表达在Ⅰ～Ⅳ级的阳性率分别为56.3%、68.2%、80.8%、100.0%,其表达随着肿瘤级别的增高而增加(χ2=9.602,P<0.05);PCNA蛋白Ⅰ～Ⅳ级的阳性率分别为37.5%、54.5%、69.2%、93.8%,其表达随着肿瘤级别的增高而增加(χ2=12.147,P<0.01)。PTTG、PCNA蛋白表达显著正相关(γs=0.557,P<0.01)。结论:PTTG、PCNA蛋白表达与胶质瘤的恶性程度密切相关,二者在胶质瘤中的发生、发展中有协同作用,并可以作为胶质瘤生物学行为的指标。Objective：To investigate the expressions and their relationship of pituitary tumor transforming gene（PTTG） and proliferating cell nuclear antigen（PCNA） in glioma. Methods：The protein expression of PTTG and PCNA were detected by immunostaining assay using Streptavidin-Peroxidase（SP） mothed in 80 cases of glioma. Results：The positive rate of PTTG in gradeⅠ～Ⅳ were 56.3%,68.2%,80.8%,100.0% respectively. The protein expression of PTTG increased with the increasing of the pathological grade（χ^2 = 9.602,P 〈 0.05）. The positive rate of PCNA protein were 37.5%,54.5%,69.2%,93.8% respectively,and the protein expression of PCNA increased with the increasing of the pathological grade（χ2 = 12.147,P 〈 0.01）. The expressions of PTTG have positive correlation with the expression of PCNA protein（γs = 0.557,P 〈 0.01）. Conclusion：The expressions of PTTG and PCNA protein were related to maligant degree of glioma, and might cooperate with eath other in the tumor genesis and progression. They should be considered as the indicators of the biological behavior in glioma.

关 键 词：垂体瘤转化基因 细胞增殖核抗原 胶质瘤 免疫组织化学 

分 类 号：R651.1[医药卫生—外科学]