仙鹤草挥发油化学成分的气相色谱-质谱分析  

Analysis of the volatile components of Agrimonla Pilosa Ledeb by gas chromatography-mass spectrometry

作　　者：李雅文[1] 黄兰芳[1] 梁晟[1] 郭紫明[2] 戴云辉[1] 吴名剑[2] 钟科军[2] 郭方遒[1] 梁逸曾[1] 

机构地区：[1]中南大学化学化工学院,湖南长沙410083 [2]常德卷烟厂技术中心,湖南常德415000

出　　处：《中南大学学报（自然科学版）》2007年第3期502-506,共5页Journal of Central South University:Science and Technology

基　　金：湖南省发展和改革委员会资助项目([2004]714);常德卷烟厂博士后科研基金资助项目

摘　　要：据化学计量学分辨方法,提出一个用气相色谱-质谱联用法定性定量分析仙鹤草中挥发油成分的方法。对重叠色谱峰采用直观推导式演进特征投影法进行分辨,从而获得每一组分的纯色谱和质谱,对每一组分纯质谱在NIST质谱库进行相似性检索并进行定性分析,用总体积积分法进行定量分析。从仙鹤草挥发油样品中共分离出56个色谱峰,鉴定出其中50个组分,占挥发油总含量的90.53%,其主要组分为醇和单萜类化合物,分别占总化合物的50.58%和18.34%。采用该方法可为进一步研究和开发仙鹤草的药用价值提供有用信息。Based on chemometric resolution methods, a method for qualitative and quantitative determination of the volatile components in Agrimonla Pilosa Ledeb was developed by chromatography-mass spectrometry. The overlapping chromatographic peaks were resolved into pure chromatograms and spectra with heuristic evolving latent projections （HELP）, qualitative analysis was performed by similar search in NIST library with the obtained pure mass spectrum of each component and the quantitative results were obtained by calculating the volume of total two-way response. The results show that 56 components are separated and 50 components are identified, accounting for 90.53% of the total content. The main components are alcohols and monoterpene, accounting for 50.58% and 18.34% of the total content, respectively. The present method can be used to provide useful information for further study and development of Agrimonla Pilosa Ledeb.

关 键 词：化学计量学 气相色谱-质谱分析 仙鹤草 挥发油 

分 类 号：O657.72[理学—分析化学]