哺乳动物细胞色素P450在大肠杆菌上表达的研究进展  

Advancement of mammalian CYP450s expression in E.coli

作　　者：张澈[1] 张志权[1] 周宏灏[2] 

机构地区：[1]湖北省郧阳医学院附属太和医院药学部 [2]中南大学临床药理研究所

出　　处：《中国临床药理学与治疗学》2007年第9期970-973,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(30371668)

摘　　要：细胞色素P450(cytochrome P450,CYP450)是人体内代谢各种内外源性物质最重要的酶系之一。随着分子生物学和重组技术的飞跃发展,对哺乳动物来源的CYP450cDNA进行精细操作并将使其在异源系统中表达成为可能。目前已经有多种异源系统被用来进行哺乳动物CYP450的表达,每一种系统都各具优缺点。哺乳动物CYP450在大肠杆菌上的表达是在实验室中的主要表达方式,但采用不同的方式表达的蛋白量不一样,而且对蛋白酶的活性也有着一定的影响;同时,蛋白纯化手段的选择也会影响CYP450表达方式的应用。本文拟对哺乳动物CYP450在大肠杆菌上的表达纯化现状和应用进行概述。Cytochrome P450（CYP450）is one of most important enzymes to metabolize endogenous and exogenous substrates in vivo.With the development of molecular biology and recombinant technology,the manipulating and heterogeneous expression of CYP450 cDNA become possible.Many kinds of heterogeneous expression systems have been generally operated nowadays,of which there are advantages and disadvantages respectively.It is usual to express mammalian CYP450s using E.coli as host in Lab,but different protein production and enzyme activity are brought by different expression systems and purification procedures.The present application of different expression modes using E.coli for mammalian CYP450 cDNA will be introduced as well as their advantages and the limits respectively.

关 键 词：异源表达 细胞色素P450 大肠杆菌 

分 类 号：R968[医药卫生—药理学]