低氧内皮细胞条件培养液和DDPH对肺动脉平滑肌细胞骨桥蛋白基因及α平滑肌肌蛋白表达的影响  

Effects of HECCM and DDPH on the expression of osteopontin gene and α-SM-actin in PASMC

作　　者：杜兵[1] 陈蓓蓓[2] 王西明[2] 段秋红[2] 何善述[2] 

机构地区：[1]温州医学院附属二院 [2]华中科技大学同济医学院

出　　处：《中国临床药理学与治疗学》2007年第9期1013-1017,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金资助(39270295)

摘　　要：目的:研究低氧内皮细胞条件培养液(HEC-CM)和DDPH对肺动脉平滑肌细胞(PASMC)骨桥蛋白(OPN)基因表达和α平滑肌肌动蛋白(α-SM-actin)的影响,进而探讨DDPH对增殖后PASMC的逆转效应。方法:利用HECCM建立猪PASMC的增殖模型;采用细胞培养、改良的四甲基偶氮唑盐比色法(MTT)、免疫细胞化学染色测定α-SM-actin、逆转录-聚合酶链反应(RT-PCR)检测OPN基因表达等方法观察HECCM的促PASMC增殖作用,以及DDPH对增殖后PASMCα-SM-actin和OPN表达的影响。结果:(1)HECCM促PASMC增殖后,PASMC的α-SM-actin含量降低而其OPN基因表达上调;(2)HECCM促PASMC增殖后,加入DDPH可使PASMC的α-SM-actin含量回升,OPN表达下调。结论:DDPH对HECCM促PASMC增殖有逆转效应,可使增殖后PASMC由合成表型逆转为正常收缩表型。AIM：To study the effects of hypoxial endothelia cell conditional medium（HECCM） and DDPH on the expression of osteopontin（OPN） gene and α-SM-actin in pulmonary arterial muscle cell（PASMC）,and the reverse effect of DDPH on proliferating PASMC induced by HECCM.METHODS：Hypoxial endothelia cell model was established in PASMC of swine.MTT assay and Immunocytochemistry was used to test cell proliferation,the expression of α-SM-actin.Reverse transcription polymerase chain reaction（RT-PCR） was used to detect the expression of OPN gene.RESULTS：（1）HECCM decrease the level of α-SM-actin and up-regulate the expression of OPN gene in PASMC;（2）DDPH increase the level of α-SM-actin and down-regulate the expression of OPN gene in PASMC.CONCLUSION：DDPH could reverse the phenotype transformation of PASMC exposed to HECCM,and transform PASMC from synthetic phenotype to normal contractile phenotype.

关 键 词：DDPH α-SM-actin 骨桥蛋白 低氧 肺动脉平滑肌细胞 

分 类 号：R965.2[医药卫生—药理学]