Analysis of both chitinase and chitosanase produced by Sphingomonas sp.CJ-5  

作　　者：ZHU Xu-fen ZHOU Ying FENG Jun-li 

机构地区：[1]College of Life Science, Zhejiang University, Hangzhou 310058, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2007年第11期831-838,共8页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the Marine Bureau of Zhejiang Province, China

摘　　要：A novel chitinolytic and chitosanolytic bacterium, Sphingomonas sp. CJ-5, has been isolated and characterized. It secretes both chitinase and chitosanase into surrounding medium in response to chitin or chitosan induction. To characterize the enzymes, both chitinase and chitosanase were purified by ammonium sulfate precipitation, Sephadex G-200 gel filtration and DEAE-Sepharose Fast Flow. SDS-PAGE analysis demonstrated molecular masses of chitinase and chitosanase were 230 kDa and 45 kDa respectively. The optimum hydrolysis conditions for chitinase were about pH 7.0 and 36 ℃, and these for chitosanase were pH 6.5 and 56 ℃, respectively. Both enzymes were quite stable up to 45 ℃ for one hour at pH 5-8. These results show that CJ-5 may have potential for industrial application particularly in recycling of chitin wastes.A novel chitinolytic and chitosanolytic bacterium, Sphingomonas sp. CJ-5, has been isolated and characterized. It secretes both chitinase and chitosanase into surrounding medium in response to chitin or chitosan induction. To characterize the enzymes, both chitinase and chitosanase were purified by ammonium sulfate precipitation, Sephadex G-200 gel filtration and DEAE-Sepharose Fast Flow. SDS-PAGE analysis demonstrated molecular masses of chitinase and chitosanase were 230 kDa and 45 kDa respectively. The optimum hydrolysis conditions for chitinase were about pH 7.0 and 36 °C, and these for chitosanase were pH 6.5 and 56 °C, respectively. Both enzymes were quite stable up to 45 °C for one hour at pH 5~8. These results show that CJ-5 may have potential for industrial application particularly in recycling of chitin wastes.

关 键 词：Sphingomonas sp. CHITINASE CHITOSANASE 

分 类 号：Q55[生物学—生物化学]