呼吸道病毒基因或抗原在激素敏感型单纯性肾病综合征肾组织和尿液的表达  

Respiratory Virus Expression in Renal Tissues and Urine of Children with Steroid Responsive Simple Nephrotic Syndrome

作　　者：吴瑾[1] 王峥[1] 王栋[1] 

机构地区：[1]四川大学华西第二医院儿科,成都610041

出　　处：《四川大学学报（医学版）》2007年第6期969-972,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号39870747);卫生部科研基金(批准号98-1-23)资助

摘　　要：目的探讨呼吸道病毒感染与激素敏感型单纯性肾病综合症(SRSNS)发病的关系。方法采用逆转录-聚合酶链反应(RT-PCR)、桥联酶标(APAAP)法,分别对38例SRSNS患儿(急性期28例,缓解期10例)、18例肾炎性肾病、15例继发性肾病、16例毛细支气管炎和15例正常对照儿童尿液呼吸道病毒基因、抗原进行检测。APAAP法检测2例SRSNS急性期患儿肾组织呼吸道病毒抗原表达。结果SRSNS急性期组尿液呼吸道病毒基因、抗原检出率均高于其他各组,差异有统计学意义(P<0.05),其中呼吸道合胞病毒检出率最高。病毒检出率在是否应用激素间的差异无统计学意义。病毒基因与抗原检测结果有较好一致性。2例SRSNS急性期患儿肾组织APAAP法分别发现流感及副流感病毒,与尿液RT-PCR、APAAP结果一致。结论呼吸道病毒可能是微小病变型肾病重要触发因素。Objective To explore the relationship between respiratory virus infection and the episode of steroid responsive simple nephrotic syndrome （SRSNS）. Methods Thirty eight children with SRSNS were recruited （28 in the active stage, 10 in the remission stage）. Sixty four children （18 with nephritic nephrosis, 16 with bronchiolitis, 15 with secondary glomerular diseases and 15 without diagnosed diseases） served as controls. Reverse transcriptase-PCR （RT-PCR） and alkaline phosphoesterase-anti alkaline phosphoesterase enzymelinked assay （APAAP） were employed to detect the viral genes and antigens in the urines respectively. The viral antigens in the renal tissues of two children with active SRSNS were also examined by APAAP. Results The viruses were more often detected in the urines of children with active SRSNS than those with remission SRSNS and the controls. The respiratory syncytial virus （RSV） was the most common virus detected in the urines of children with active SRSNS. The appearance of the viruses gene and antigens was not influenced by the use of steroid. The same antigens were found in the renal tissues of the two children with active SRSNS. Conclusion Respiratory tract viruses may play an important role of triggering the SRSNS.

关 键 词：肾病综合征 呼吸道病毒 肾脏 尿液 

分 类 号：R726.9[医药卫生—儿科]