Cu^(2+)对马尾松根尖细胞的染色体损伤研究  

Study of Effects of Cu^(2+) on Chromosomal Injury of Root Tip Cells in Pinus massoniana

作　　者：吴若菁[1] 林子都 文家友 严钦凤 陈岩松[1] 贾晗[1] 

机构地区：[1]福建师范大学生命科学学院,福州350007 [2]福建省闽侯县林业局祥谦林业站,福建闽侯350100 [3]福建省晋江市东石中学,福建晋江362200 [4]福建省晋江市磁灶中学,福建晋江362200

出　　处：《亚热带资源与环境学报》2007年第3期49-54,共6页Journal of Subtropical Resources and Environment

基　　金：福建省教育厅科学研究基金资助项目(JB04231)

摘　　要：利用细胞遗传学技术,研究Cu2+对马尾松根尖细胞遗传损伤效应.实验结果表明:Cu2+会导致马尾松根尖细胞产生遗传损伤,出现微核、染色体断片和落后染色体.随着Cu2+浓度的提高,马尾松根尖细胞的微核率和染色体分离异常率不断上升,在浓度为10.0 mg.L-1时到达最高峰;处理时间在24 h时,微核率和染色体分离异常率到达最高.此外,还分析了微核率和染色体分离异常率的相关关系,发现采用Linear模式二者的相关性最佳,此时r=0.909,二者的相关性达到显著相关水平.The technology of cytogenetics was adopted to research into the effects of Cu^2＋ on chromosomal injury of root tip cells in Pinus massoniana. The results showed that Cu^2＋ did induce heredity injury of root tip cells in Pinus massoniana, which led to the emergence of micronucleus, chromosome fragment and chromosome lagging. And, while Cu^2＋ concentration increased, the micronucleus rate and the chromosomal separation abnormalities rate both gradually rose in root tip cells. When the concentration of Cu^2＋ was 10.0 mg ·L^-1 , the value of both rates achieved peak. While processing time was 24 h, the rates got to the maximum. Furthermore, the correlation between micronucleus rate and chromosomal separation abnormalities rate was analyzed, which showed that, when using the Linear mode, the best correla- tion rate would be r=0. 909 over significant level.

关 键 词：CU^2+ 马尾松 染色体损伤 相关分析 

分 类 号：Q947.8[生物学—植物学]