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机构地区:[1]大连水产学院农业部海洋水产增养殖学与生物技术重点开放实验室,辽宁大连116023 [2]中国科学院海洋研究所,山东青岛266071
出 处:《水产学报》2007年第6期853-859,共7页Journal of Fisheries of China
基 金:中国科学院知识创新工程课题(ZKCX2-211);国家"八六三"高新计划(2005AA603610)
摘 要:菲律宾蛤仔(Ruditapes philippinarum)是我国重要海水滩涂增养殖贝类,在福建、山东、辽宁等地已成为水产养殖的重要支柱产业。我国北方菲律宾蛤仔苗种来源主要靠天然苗种和从南方购进,受自然条件严重制约;在南方,主要依靠室外土池育苗,产量不稳定。因此,研究人工促熟条件下不同海区菲律宾蛤仔性腺发育组织学等繁殖生物学特性,对解决苗种短缺问题的根本途径——室内全人工育苗具有重要的意义。Manila clam, Ruditapes philippinarum, is one of the most important clam species cultured in China. By observing gonadal development of Manila clam from different areas under controlled water temperatures, we could forecast and control the spawning time. Manila clam was sampled in Dalian and Fujian in March,2004. They were warmed up in indoor ponds to ripe with the same condition. Shell size and weight were measured. We researched the histology of its gonad develepment and the relationship between periodic variation of histology with gonad development and effective accumulated temperature. The gonads were fixed with Bouin's solution, and tissue sections were stained with H. E solution. Taking micrography pictures to show the gonad development of the Manila clam in Dalian was divided into five sections:spent stage, early active stage, later active stage, mature stage, spawned stage. The gonad development of the Manila clam in Fujian was also divided into five sections:early active stage, later active stage, mature stage, spawned stage, degenerating stage. The relationship of gonad develepment with effective accumulated temperature was discussed in this paper. It indicated that the effective accumulated temperatures of gonad development of the Manila clam in Fujian and Dalian were 190.1 ℃.d and 331.8 ℃.d,respectively.
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