组胺H_2受体拮抗剂促进人胃粘膜上皮细胞的增殖和迁移  

Histamine H 2 receptor antogonist promotes proliferation and migration of human gastric epithelial cells

作　　者：陈学清[1,2] 张万岱[1,2] 陈阳述 宋于刚[1,2] 王继德[1,2] 周殿元 

机构地区：[1]第一军医大学南方医院消化内科 [2]广州空军医院肝病研究所

出　　处：《新消化病学杂志》1997年第6期357-358,共2页

摘　　要：目的评价二盐酸组胺和组胺Ｈ２受体拮抗剂西咪替丁对人胃粘膜上皮细胞ＧＥＳ１增殖和迁移的影响．方法细胞增殖检测采用酸性磷酸酶细胞原位计数法．细胞迁移用细胞损伤检测法．同时，用Ｆｌｕｏ３ＡＭ为荧光标记物在粘附式细胞仪上进行了细胞内游离钙测定．结果西咪替丁浓度为１０５，１０６，１０７，１０８和１０９ｍｏｌ／Ｌ时，细胞数分别比对照组增加５８２％、６００％、７３７％和７４５％（Ｐ＜００１），细胞迁移数（１０６ｍｏｌ／Ｌ）为对照组的５９２％（Ｐ＜００５）；而组胺对细胞的增殖和迁移无明显影响．西咪替丁刺激可使ＧＥＳ１细胞内（Ｃａ２＋）ｉ浓度呈双向改变．结论西咪替丁可促进体外人胃粘膜上皮细胞的增殖和迁移，这可能是Ｈ２受体拮抗剂抗溃疡的机制之一，其途径可能部分是通过钙离子信号系统介导的．AIM To investigate the effect of histamine H 2 receptor antagonist on the proliferation and migration of human gastric epithelial cells GES 1. METHODS The number of cell in situ were counted by acid phosphate substrate method, cell migration by cell wound assay, and dynamic change of cytosolic (Ca 2+ ) of GES 1 cells with Fluo 3 AM indicator in ACAS 570 interactive laser cytometer. RESULTS ①Histamine did not affect the migration and proliferation as well as the cytosolic (CA 2+ )i of GES cells; ②Compared with untreated and histamine groups, cell migration was increased by 59 2% at a concentratin of 10 -6 mol/L ( P <0 05), cell proliferation was increased by 58 2%, 60 0% 73 7% and 74 5% at a concentration of 10 -5 , 10 -6 , 10 -7 and 10 -8 mol/L, respectively ( P <0 01) while GES 1 cells were stimulated with cimetidine; ③A biphase rise in (Ca 2+ ) representing transient increase of cytosolic (Ca 2+ )i at earlier stage and afterward persistent extracellular (Ca 2+ ) influx were observed in GES 1 cells stimulated by 10 -6 mol/L cimetidine. CONCLUSION Cimetidine could promote migration and proliferation of gastric mucosal cells in vitro and the mechanism might be related to calcium messenger system. Ulcer healing property of H 2 antagonist in vivo would be dependent partly on promoting migration and proliferation of gastric mucosal cells.

关 键 词：胃粘膜 消化性溃疡 西咪替丁 组胺H2受体 拮抗剂 

分 类 号：R975.6[医药卫生—药品] R573.105[医药卫生—药学]