Se和VE对大鼠心肌肌球蛋白重链基因表达影响的研究  

EFFECTS OF SELENIUM AND VITAMN E ON EXPRESSION OF CARDIAC MYOSIN HEAVY CHAIN GENE IN RATS

作　　者：李荣文[1,2,3] 朱筱娟 刘晓茹[1,2,3] 李绍巍[1,2,3] 杨同书 

机构地区：[1]白求恩医科大学应用基础医学研究所 [2]解放军农牧大学 [3]白求恩医科大学预防医学院

出　　处：《营养学报》1997年第1期1-6,共6页Acta Nutrimenta Sinica

基　　金：国家自然科学基金

摘　　要：从克山病病区粮和非病区粮及病区粮补加Ｓｅ和ＶＥ喂养的大鼠心肌中提取总ＲＮＡ，采用斑点印迹法分析其中的α和β－心肌肌球蛋白重链（ＣＭＨＣ）ｍＲ－ＮＡ含量的变化，并对大鼠血清及心肌组织中的Ｔ４、Ｔ３含量及Ｔ４５－脱单碘酶活性进行了观测。结果表明，与对照组相比，病区粮喂养大鼠心肌组织中的α－ＣＭＨＣｍＲＮＡ含量降低而β－ＣＭＨＣｍＲＮＡ含量升高，同时心肌和血清中Ｔ３含量显著下降，血清Ｔ４增高而心肌Ｔ４变化不大，心肌中Ｔ４５－脱单碘酶活性降低。补加Ｓｅ和ＶＥ后上述改变明显回转，提示克山病致病因素可导致大鼠ＣＭＨＣ基因表达的偏移。Ｓｅ和ＶＥ对心肌肌球蛋白的保护作用可能与心肌Ｔ４５－脱单碘酶活性、Ｔ３含量及其对ＣＭＨＣ基因表达的调控有关。Total RNA was extracted from myocardium in rats fed with Keshan disease area grains (endemic grains, EG)and in those fed with EG+Se(0.1μg/g);EG+VE(100μg/g);EG+Se(0.1μg/g)+VE(100μg/g);Non EG as well as stock diet. The cDNA of α and β Cardiac Myosin Heavy Chain (CMHC) labeled with biotin was used as gene probes to detect α and β CMHC mRNA content in rat myocardium by using the dot blot. The results showed that two months after treatment, α CMHC mRNA level was significantly lower and β CMHC mRNA level was significantly higher in myocardium of EG group as compared with those of Non EG group (P<0.05). The concentrations of T 3 in serum and myocardium and T 4 5 monodeiodinase in myocardium of EG group were decreased, but the concentration of T 4 in serum was increased, in addition, a reversible alteration was found after adding Se or/and VE. The results indicate that some pathogenic factors of Keshan disease may alter the expression of the CMHC gene, whereas the Se and VE may have a reversible effect on this change caused by the pathogenic factors. The protective effect of Se and VE on cardiac myosin my be related to T 4 5 monodeiodinase, T 3 concentration in myocardium and expression of the CMHC gene.

关 键 词：硒 维生素E 心肌 肌球蛋白重链 基因表达 克山病 

分 类 号：R542.3[医药卫生—心血管疾病]