表棓儿茶素棓酸酯脂质纳米粒制备及含量测定  

Preparation and determination of epigallocatechin gallate solid lipid nanoparticles

作　　者：潘桂玲[1] 刘颖[2] 陈祥峰 

机构地区：[1]徐州医学院附属医院,徐州221000 [2]南京工业大学制药与生命科学学院,南京210009 [3]南京海光应用化学研究所,南京211112

出　　处：《药学与临床研究》2007年第5期429-432,共4页Pharmaceutical and Clinical Research

摘　　要：目的：制备表梧儿茶素桔酸酯固体脂质纳米粒并测定其含量。方法：用正交试验法优选处方的组成；用HPLC法测定EGCG的含量，选用LichrospherC18柱（4．6mm×250mm），以甲醇-水（30：70）为流动相，检测波长278nm，流速1．0mL·min^-1。结果：最佳处方为大豆卵磷脂0.2094g，胆固醇0.5578g，表梧儿茶素掊酸酯0．0767g，纯净水30mL；所制得表梧儿茶素梧酸酯固体脂质纳米粒粒径在60-200nm之间，平均粒径120nm，包封率达79．8％，载药量为7．0％。结论：本方工艺可行；建立的高效液相色谱法简便、准确。Objective:To prepare epigallocatechin gallate(EGCG) solid lipid nanoparticles(SLN) and determine the EGGC content in SLN.Method: Orthogonal experiment design was used to optimize the prescription of SLN.The HPLC method was used with a Lichrospher C18(4.6 mm×250 mm) column and a mobile phase consists of methanol-water(30∶70) at the detection wavelength of 278 nm and the flow rate of 1.0 mL·min-1.Result: The best prescription of SLN are: lecithin 0.209 4 g,cholesterol 0.557 8 g,EGCG 0.076 7g and pure water 30 mL.The average diameter of prepared SLN is 120 nm.The entrapment efficiency is 79.8% and ratio of loading drug is 7.0%.Conclusion: The prescription of EGCG SLN is feasible and the established HPLC method is simple,accurate and reliable.

关 键 词：表掊儿茶素梧酸酯 固体脂质纳米粒 制备工艺 高效液相色谱法 含量测定 

分 类 号：TQ460.6[化学工程—制药化工] R927.2[医药卫生—药学]