丙丁酚对氧化型低密度脂蛋白损伤内皮的保护作用  

Probucol Prevents the Cultured Endotbelial Cells from Injury Induced by Oxidized Low Density Lipoprotein

作　　者：沈成兴[1] 罗俊 刘乃丰[1] 陈日新[1] 

机构地区：[1]南京铁道医学院附属第一医院心内科,南京210009 [2]南京市立一医院心内科

出　　处：《中国动脉硬化杂志》1997年第1期52-54,共3页Chinese Journal of Arteriosclerosis

摘　　要：用培养的人脐静脉内皮细胞为对象，用＾３Ｈ－ＴｄＲ掺入法，观察了抗氧化剂丙丁酚对氧化型低密度脂蛋白作用下的内皮细胞ＤＮＡ合成的保护作用，并用扫描电子显微镜观察其对培养细胞形态结构的影响。结果发现，在作用２４ｈ后，氧化型低密度脂蛋白组的＾３Ｈ－ＴｄＲ掺入量是天然低密度脂蛋白组的４８％，是丙丁酚组的５６％，超微结构显示丙丁酚组的细胞结构保持正常，而氧化型低密度脂蛋白组则受到破坏。Aim Endothelial cells injured by free radicals is a crucial step in tbe development of many diseases. We investigated the protective effect of probucol. an an-tiatherosclerosis drug,to the cultured hurnan endothe-lial cells.Methods The oxidized low density lipoprotein (OLDL) were used as free radial resources and 'H-TdR incorporation of the cells was estimated as DNA sgnthesis. The microstructure change was studied by using the scanning electron misroscope.Results At 24 hours interval, the k-TdR incorPo-rated into the cells in OLDL group was 48K as that of nLDL group or 56K as that of probucol + OLDL group (P<0.001 ). The scanning electron micro-scopic study showed that the intercellular junctions di-ndnished and the cellular outline changed in the OLDL treated cells but not the probucol treated cells.CoarIasloas Probucol is an useful antioxidant in preventing the cultured cells from injury by free radi-cals and perhaps tbus contributes to the antiathero-genesis effeCt.

关 键 词：丙丁酚 低密度脂蛋白 抗氧化剂 动脉粥样硬化 

分 类 号：R965[医药卫生—药理学] R543.505[医药卫生—药学]