南极嗜冷假单胞菌7197的分离和无机焦磷酸酯酶（PPase）基因ppa的克隆  

Isolation of antarctic psychrotrophilic Pseudomonas sp. 7197 and cloning of ppa gene for inorganic pyrophosphatase （PPase）

作　　者：张金伟[1] 曾润颖[1] 

机构地区：[1]国家海洋局第三海洋研究所海洋生物遗传资源重点实验室,厦门361005

出　　处：《高技术通讯》2007年第10期1067-1071,共5页Chinese High Technology Letters

基　　金：国家自然科学青年基金（40406029）资助项目.

摘　　要：从南极普利兹湾深海沉积物中筛选到一株耐冷菌株7197。16S rDNA序列分析表明,该菌株属于假单胞菌属（Pseudomonas）。从该菌的全基因组DNA中克隆到编码无机焦磷酸酯酶（PPase）的ppa基因完整的开放阅读框（ORF）,其全长为531bp。该基因编码一个由176AA残基组成的分子量预计为19631 Da的PPase蛋白质,其氨基酸序列与Psy-chrobacter sp.273-4的PPase有97%的相似性,与Neisseria meningitidisZ2491的PPase有79%的相似性,与Mannheimia succiniciproducens MBEL55E的PPase有75%的相似性。A psychrotrophile strain 7197, which produces inorganic pyrophosphatase （PPase）, was isolated from the deep sea sediment of Prydz Bay, Antarctic. The morphology identification and 16S rDNA sequence analysis showed that the strain belongs to genus Pseudomonas. The ppa gene for inorganic pyrophosphatase （PPase） was cloned by PCR according to the primers. Nucleotide sequence analysis revealed an open reading frame （ORF） of 531 bp encoding the PPase. The amino acid sequence deduced from the nucleotide sequence of the ppa corresponded to a protein of 176 amino acid residues with a molecular weight of 19531 kDa. The PPase showed the highest amino acid sequence identity to the PPase of Psy- chrobacter sp. 273-4 with 97%, and had 79% and 75% of identity to the PPase of Neisseria meningitidis Z2491 and Mannheimia succiniciproducens MBEL55E respectively.

关 键 词：南极 耐冷菌 假单胞菌属 无机焦磷酸酯酶 

分 类 号：Q781[生物学—分子生物学]