南瑞苕AFLP指纹图谱的构建与聚类分析  

Construction and analysis of AFLP fingerprinting in Nancy holls of sweetpotato

作　　者：蒲志刚[1] 王大一[2] 谭文芳[2] 梁勤[1] 吴洁[1] 钟婷婷[1] 阎文昭[1] 

机构地区：[1]四川省农业科学院生物技术核技术研究所,四川成都610066 [2]四川省农业科学院作物研究所,四川成都610066

出　　处：《西南农业学报》2007年第5期1085-1087,共3页Southwest China Journal of Agricultural Sciences

基　　金：四川省应用基础项目(07JY029-104);四川省育种攻关专项资金项目(2006YZGG01-002)

摘　　要：以甘薯生产中主要应用的亲本材料南瑞苕及其子代品种为材料,用建立的AFLP分子标记体系进行研究,筛选得到5对多态性高、分辨力强的引物EATT、MCCA等,通过这5对引物构建出了南瑞苕的指纹图谱。用16对引物对47个与南瑞苕相关的品种进行聚类分析,统计DNA多态性条带后用UPGMA方法获得了聚类分析图,根据结果将47个品种分为5大类型,该实验结果为甘薯育种工作者了解各个品种的相互关系和进一步应用某一材料的优良性状选育品种奠定了基础。AFLP（Amplified Fragment Length Polymorphism） analysis system was established in Nancy holls that used widely sweetpotato breeding in China.These 5 pairs of primers like EATT,MCCA which had high polymorphism and powerful distinctiveness were been selected from 16 pairs of primers to establish the fingerprinting of Nancy holls.16 pairs primers were used for clustering analysis about 47 related varieties with Nancy holls.The genetic distance of Nancy holls was calculated and its dendrogram which were divided into 5 types was performed based on AFLP data of 16 pairs of primers.According to these results,the breeders could know and use Nancy holls better.

关 键 词：甘薯 南瑞苕 AFLP DNA指纹图谱 聚类分析 

分 类 号：S531[农业科学—作物学]