青白杨派间杂交的胚珠培养研究  

Ovule Culture of Leuce-Tacamahaca Hybrids

作　　者：卫尊征[1] 张金凤[1] 马海渊[1] 郭军 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]河南省泌阳县马谷田镇农业技术推广中心

出　　处：《防护林科技》2007年第6期19-20,34,共3页Protection Forest Science and Technology

基　　金：北京林业大学研究生自选课题基金资助

摘　　要：对由8个白杨派基因型和5个青杨派基因型所组成的13个青白杨派间杂交组合进行胚珠培养试验研究。结果表明:三交要明显好于单交;培养基对胚珠培养影响不大,较合适的培养基为1/2MS+6-BA 1.0 mg.L-1+NAA 1.0 mg.L-1;基因型TB03做母本培养效果优于TB04;随着授粉时间延长,胚珠萌发率也越高。Ovule culture of 13 intersectional hybrids produced from 8 genotypes of Leuce and 5 genotypes of Tacamahaca were studied. The results show that triple cross is better than single cross. Medium has little effects on ovule culture. Optimal medium is 1/2MS ＋6 -BA 1.0mg · L^-1 ＋NAA 1.0 mg · L^-1. Effect of female parent TB03 ovule germinates is well than genetype TB04; ovule germination rate increases along with the day increase after pollination.

关 键 词：青杨 白杨 派间杂交 胚珠培养 

分 类 号：S722.34[农业科学—林木遗传育种]