川芎嗪抗大鼠免疫损伤性肝纤维化作用及机制研究  

Study of tetramethylpyrazine in treatment on liver fibrosis and mechanism in rats

作　　者：华海婴[1] 李艳瑛[2] 戈士文[1] 

机构地区：[1]郑州大学医药科学研究院,郑州450052 [2]黄河三门峡医院药剂科,三门峡472000

出　　处：《中药药理与临床》2007年第5期60-62,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河南省院所预研专项基金资助课题(0641190109)

摘　　要：目的:观察川芎嗪(TMP)对大鼠免疫损伤性肝纤维化的治疗作用并探讨其相关机制。方法:建立猪血清诱导的大鼠免疫性肝纤维化模型后给予TMP干预,分别观察TMP对受试动物肝组织病理学分级、血清生化和血清纤维化指标的影响,免疫组化法检测TGFβ-1、TIMP-1在肝组织的阳性表达。结果:TMP 10、20和40mg/kg均可显著改善大鼠肝纤维化分级程度;显著降低大鼠血清ALT、AST、ALP水平,提高A/G比值;下调TGFβ-1、TIMP-1表达;TMP各剂量可不同程度的降低肝纤维化大鼠血清HA、LN、C-IV和PⅢNP水平。结论:TMP对大鼠实验性肝纤维化具有明显的治疗作用;其机制可能与抑制细胞因子TGFβ-1、胶原酶抑制因子TIMP-1表达增高有关。Objective： To investigate the effects of tetramethylpyrazine （TMP） for chronicity liver fibrosis in rats and the possible mechanism . Methods： A model of liver fibrosis was set up by intraperitoneal injection porcine serum into rats. The effect of TMP（ 10,20 and 40mg/kg） in the treatment of liver injury was evaluated by the degree of liver fibrosis, the serum biochemical indicator included ALT, AST and ALP, and fibrosis indicator included serum HA, LN,C-Ⅳ and PⅢNP. The expression of transforming growth factor-beta 1 （TGF-β1）, tissue inhibitor of metalloproteinase-1 （ TIMP-1 ） in hver tissue were detected by immunohistochemical techniques. Results： The degree of liver fibrosis indexes were improved significantly; the serum ALT, AST, ALP level were depressed but the level of A/G were enhanced; the expression of TGF-β1, TIMP-1 were reduced by TMP. The serum HA, LN, C-Ⅳ and PⅢNP level in model rats were decreased by TMP. Conclusion： TMP has treament actions to rats with immunodamaged hepatic fibrosis. The mechanism of its effect is related with its inhibition the expression of TGF-β1 and TIMP-1 in rats liver.

关 键 词：川芎嗪 肝纤维化 转化生长因子Β1 金属蛋白酶组织抑制因子-1 

分 类 号：R285.5[医药卫生—中药学]