雄黄及含雄黄复方对炎症介质IL-1β、IL-6、TNF-α和NO的影响  

Effects of Realgar and prescriptions containing Realgar on inflammatory mediators IL-1β,IL-6,TNF-α and NO

作　　者：汤毅珊[1] 王宁生[1] 张银卿[1] 

机构地区：[1]广州中医药大学临床药理研究所,广州510405

出　　处：《中药药理与临床》2007年第5期107-110,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(30672637)

摘　　要：目的:研究雄黄和含雄黄复方对病理状态下(感染性脑损伤模型、发热模型)炎症介质IL-1β、IL-6、TNF-α和NO的影响,探讨雄黄的"解毒"作用机理。方法:用百日咳菌造成感染性大鼠脑损伤模型,用酵母菌造成大鼠发热模型,取血用ELISA法测定血清中的炎症细胞因子IL-1β、IL-6和TNF-α的水平,比色法测定脑组织、血清中一氧化氮合酶(NOS)及其同工酶(诱导型一氧化氮合酶iNOS)的活力。结果:雄黄和安宫牛黄散均可显著降低脑损伤大鼠血清IL-1β、IL-6和TNF-α水平,其中IL-1β回复到正常水平,IL-6和TNF-α的降幅随雄黄剂量的增大而增大;雄黄和安宫牛黄散均可显著抑制脑组织中NOS和iNOS的活力,复方的抑制作用大于雄黄。雄黄和牛黄解毒片可显著降低发热大鼠血清IL-1β水平,药后4h基本回复到正常水平;药后2h牛黄解毒片可显著降低血清NOS和iNOS的活力。结论:抑制病理状态下过度释放的炎症介质(IL-1β、IL-6、TNF-α和NO)可能是雄黄在复方中发挥"解毒"功效的途径之一。Objective： To explore the pharmacological mechanism of Realgar by way of studying the effects of Realgar and prescriptions containing Realgar on inflammatory mediators IL - 1β, IL - 6, TNF - α and NO under pathologic status（ infectious cerebral injury model and fever model）. Methods： In experiment 1, SD rats were randomly divided into six groups, and 6 rats in each group： untreated normal group, infectious cerebral injury model group, model rats administrated by Realgar of low, middle, high dosage（75mg/kg, 150mg/kg, 300mg/kg）and Angong Niuhuang powder（ANP, 1.5g/kg）. Blood samples and brain tissues were collected at 3 h after administration. In experiment 2, SD rats were randomly divided into four groups, and 15 rats in each group： untreated normal group, fever model group, model rats administrated by Realgar（90mg/kg）and Niuhuang Jiedu Tablet（ NJT, 1. 404g/kg）. Each group was divided into three subgroups（5 rats/subgroup）. Blood samples of the rats in subgroups were collected at 1 h, 2h and 4h after administration respectively. ELISA method was used to determine inflammatory cytokines（ IL- 1β, IL- 6 and TNF- α） levels in serum. Spectrophotometry was used to test activities of nitric oxide synthetase （NOS） and its isoenzyme（inducible nitric synthetase, iNOS） in serum and brain tissues. Result： Realgar and ANP significantly decreased levels of IL - 1β, IL - 6 and TNF - α in cerebral injury rat serum as compared with the model group. Particularly, the level of IL - 1β recovered to normal level. The decrease ranges of IL - 6 and TNF - α rose with the Realgar dose. Realgar and ANP significantly inhibited the activities of NOS and iNOS in rat brain as compared with the model group. The decrease range of iNOS activity in ANP group was significantly higher than those in three Realgar groups. Realgar and NJT significantly decreased the level of IL - 1β in fever rat serum as compared with the model group, and the level of IL - 1β recovered to normal level at 4hrs after

关 键 词：雄黄 安宫牛黄散 牛黄解毒片 白介素-1Β 白介素-6 肿瘤坏死因子-α 一氧化氮 感染性脑损伤 发热 

分 类 号：R285.5[医药卫生—中药学]