鸭胚胎代用蛋壳培养的研究  

Culture of Duck Embryo in Surrogate Eggshells

作　　者：张文昌[1] 徐国正[1] 庄益芬[1] 王垚锟[1] 

机构地区：[1]福建农林大学动物科学学院,福州350002

出　　处：《中国农学通报》2007年第11期8-11,共4页Chinese Agricultural Science Bulletin

基　　金：福建省教育厅高校科学技术研究项目"鸭体外受精和胚胎移植的研究"(JA05245)

摘　　要：采用两种方法进行了鸭胚胎代用蛋壳培养,旨在提高出雏率。方法Ⅰ将新鲜种蛋的胚胎转移至代用蛋壳Ⅰ内培养,72h后转移至代用蛋壳Ⅱ内培养至出雏。方法Ⅱ将正常孵化72h后的胚胎转移至代用蛋壳Ⅱ后培养至出雏。方法Ⅰ组出雏率为19.2%(10/52);方法Ⅱ组出雏率为40.4%(21/52)。从第6天到出雏同一日龄胚胎存活率方法Ⅰ组显著低于方法Ⅱ组和对照组(P<0.05或P<0.01)。第16天到出雏同一日龄胚胎存活率方法Ⅱ组显著低于对照组(P<0.01)。结果表明方法Ⅰ和方法Ⅱ两种方法均能获得雏鸭,方法Ⅱ优于方法Ⅰ。To increase the hatchability in vitro, two methods were used to culture the duck embryos in surrogate eggshells. Method one： embryos were transferred into the surrogate eggshell I from the freshly laid eggs, after being cultured for 72hrs, the embryos were transferred into the surrogate eggshell Ⅱ until hatching. Method two： normal embryos, being incubated for 72 hrs, were transferred into the surrogate eggshell Ⅱ till to hatching. The results follow： The hatchability of method one and method two was 19.2% （10/52） and 40.4% （21/52）, respectively. From 6th day to hatching, the survival rate of embryos with the same cultured time in method one was significantly lower than method two and control （P〈0.05 or P〈0.01）. From 16th day to hatching, the survival rate of embryos with the same cultured time in method two was significantly lower than control （P〈0.01）. The results indicated that both of the methods can work for the embryo development in vitro, but method two is better.

关 键 词：鸭胚胎 代用蛋壳培养 存活率 

分 类 号：S814.8[农业科学—畜牧学]