植物表达载体pGMAR—BADH的构建  

Construction of the Plant Expression Vector pGMAR-BADH

作　　者：朱永兴[1] 许兴[1] 陈受宜[2] 

机构地区：[1]宁夏农业生物技术重点实验室,银川750002 [2]中国科学院遗传与发育研究所,北京100101

出　　处：《中国农学通报》2007年第11期49-51,共3页Chinese Agricultural Science Bulletin

基　　金：国家973项目"重要耐盐耐低温基因的生物学整和效应研究"(2006CB100106)

摘　　要：将甜菜碱醛脱氢酶基因BADH和植物表达载体pGMAR通过内切酶BamHI和KpnI双酶切,T4连接酶进行连接反应。重组质粒在大肠杆菌菌株DH5α内扩增,提取并纯化质粒。经鉴定,基因BADH已被完整、正确的插入到pGMAR载体中,并成功将BADH插入到载体pGMAR的两个MAR序列之间。Both the plant expression vector PGMAR and the Salt-tolerant Correlative Gene BADH in Atriplex were digested by BamHI and KpnI. Two digested fragments were ligated with T4DNA ligase and PGMAR-BADH plasmid was constructed, After the identification of digesting and sequencing, the reconstructive plasmid was confirmed that contained the correct and full nucleotides sequence of BADH in PGMAR.

关 键 词：甜菜碱醛脱氢酶基因 大肠杆菌 限制性核酸内切酶 电泳 

分 类 号：S511[农业科学—作物学]