墨西哥落羽杉离体培养及再生体系的建立  

In Vitro Culture and Plant Regeneration System of Taxodium mucronatum

作　　者：许秀玉[1] 施季森[1] 席梦利[1] 陈建新[2] 沈烈英 戴永梅 

机构地区：[1]南京林业大学国家林业局林木遗传和基因工程重点实验室,南京210037 [2]广东省林业科学研究院,广州510520 [3]上海市林业站,上海200072

出　　处：《林业科学》2007年第10期40-44,共5页Scientia Silvae Sinicae

基　　金：上海科技兴农项目"杂交墨西哥落羽杉快繁技术"部分内容

摘　　要：以墨西哥落羽杉的幼苗为起始外植体，研究其离体培养及再生体系的建立。结果表明：幼苗在DCR＋BA0．5mg·L^-1＋IBA0．2mg·L^-1培养基上诱导分化效果最好，分化频率80％以上，平均每个外植体产芽2～5个；诱导的丛生芽转入DCR＋Gln 500mg·L^-1培养基中伸长效果最佳；生根培养基为3／4DCR＋0．05％AC，生根率在75％以上。Young seedlings developing from the seeds were cultured as the initiation explants to investigate the micropropagation of Taxodium mucronatum. Cultured young seedlings on DCR medium supplemented with 0.5 mg·L^- 1 BA and 0.2 mg·L^- 1 IBA was the best way of inducement and differentiation, with inducement rate higher than 80 %, mean number of induced buds around 2～ 5. For elongation of the induced buds, the suitable culture medium was DCR ＋ Gin 500 mg·L^- 1. The medium of 3/4 DCR ＋ 0.05 % AC was used for rooting, and the rooting rate was more than 75 %. Using hormone to induce roots could produce mass of callus on the end top of cuttings and it was not benefit to the plantlets survival after transplanting. In addition, it was easy for the plantlets with long internode and vigorous stem to induce root system. When the roots developed to 2 ～4 cm long, the plantlet could be transplanted after 3～ 7 days acclimation.

关 键 词：墨西哥落羽杉 离体培养 植株再生 

分 类 号：S718.46[农业科学—林学] Q943.1[生物学—植物学]