太湖流域粳稻地方品种黑壳子粳抗稻瘟病基因的分子定位  

Molecular Mapping of Rice Blast Resistance Gene in a japonica Landrace Heikezijing from the Taihu Lake Area,China

作　　者：李培富[1] 史晓亮[1] 王建飞[1] 刘超[1] 张红生[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《中国水稻科学》2007年第6期579-584,共6页Chinese Journal of Rice Science

基　　金：国家高技术研究发展计划(863计划)资助项目(2006AA10Z162)

摘　　要：以广谱、高抗稻瘟病的太湖流域粳稻地方品种黑壳子粳与感病品种苏御糯杂交,产生F1、F2、F2∶3及F5∶6重组自交系群体,用日本稻瘟病鉴别菌系北1接种鉴定。黑壳子粳对北1的抗性是由1对显性主效基因控制的,定名为Pi-hk1(t)。根据不同杂交世代群体对北1的抗、感反应,结合SSR分子标记,将黑壳子粳中的Pi-hk1(t)基因定位在水稻第11染色体长臂末端,与RM7654和RM27381两个标记的遗传距离分别为0.9cM和1.6cM。A japonica landrace Heikezijing from the Taihu Lake area, with high resistance to rice blast （Magnaporthe grisea）, was crossed with a susceptible variety Suyunuo to get F1 , F2 and F2,3 populations. The recombined inbred lines F5, 6 were obtained from F2 generation by means of single seed descendant method. The parents, F1 , F2, F2.3 and F5, 6 populations were inoculated with Japanese blast strain Hoku 1 to illustrate the inheritance of resistance to the rice blast in Heikezijing. The resistance to Hoku 1 in Heikezijing was controlled by one dominant gene; tentatively designated as Pi-hkl （t）. The gene Pi-hkl （t） was further mapped based on the reaction to the rice blast in various generations, combined with SSR molecular markers. It was located on the end of long arm of chromosome 11 and linked to the markers RM7654 and RM27381, with a distance of 0.9 cM and 1.6 cM, respectively.

关 键 词：水稻 地方品种 稻瘟病菌 抗性遗传 基因定位 

分 类 号：S511.22[农业科学—作物学]