EPO多克隆抗体抑制EPO促CFU-E形成研究  

The Effects of Polyclonal Antierythropoietin Antibody on Simulating Colony-forming Unit-erythroid

作　　者：黄学文[1] 牟信兵[1] 高钰琪[1] 李素芝[2] 黄跃[2] 黄文超[2] 陈有[2] 孙泽平[2] 洪勇[2] 石泉贵[2] 郭俊[2] 潘丽[2] 杨定周[2] 

机构地区：[1]第三军医大学高原军事医学系,430038 [2]西藏军区总医院高山病科,850003

出　　处：《高原医学杂志》2007年第3期1-3,共3页Journal of High Altitude Medicine

基　　金：拉萨市科技局资助合作项目;项目编号:2004078

摘　　要：目的:探讨EPO多克隆抗体是否具有对抗EPO促红系形成的功能。方法:对大鼠骨髓进行定向体外红系集落形成单位(CFU-E)培养,观察CFU-E数量,判定EPO多克隆抗体对红系增殖活性的影响。结果:与常规培养组比较,EPO多克隆抗体培养组CFU-E明显减少,P<0.01,差别有非常显著性。结论:EPO抗体可抑制EPO促红系形成,其可作为治疗高原红细胞增多症病人的药物进一步研究。Objective： To observe whether the polyclonal antierythropoietin antibody against stimulating colony-forming unit-erythroid（CFU-E） in the cultured marrows from mouse. Methods： Marrows were collected from mouse, they were exposed to Lhasa from Chongqing for 3 months, and the mononuclear cells in the marrows were isolated for erythroid oriented culture. Then the level of CFU-E formation was recorded under the therapeutic condition with erythropoietin （EPO） or EPO and polyclonal antierythropoietin antibody. Results：CFU-E formation was significantly suppressed by polyclonal antierythropoietin antibody. Conclusions： antierythropoietin antibody is able to inhibit the EPO activity stimulating colony-forming unit-erythroid proliferation.

关 键 词：促红细胞生成素 抗体 红系集落形成单位 

分 类 号：R55[医药卫生—血液循环系统疾病]