喜热噬油芽胞杆菌代谢产生表面活性剂的研究  

Study on Physico-chemical Properties of the Biosurfactant Produced by Geobacillus thermoleovorans

作　　者：王凤兰[1] 王晓东[1] 

机构地区：[1]中国地质大学能源系

出　　处：《微生物学杂志》2007年第5期23-28,共6页Journal of Microbiology

摘　　要：采用丙酮抽提、高压液相分离纯化等技术从嗜热菌Geobacillus therm oleovorans以正十六烷为碳源培养的发酵液中分离获得性能突出的表面活性物质。利用甲脂化、乙酰化衍生技术结合GC-MS,MS(ESI)等鉴定该表面活性剂为单脂肪酸甘油脂。实验条件下,该表面活性剂使水的表面张力降低到32.7 mN/m,测定其临界胶束浓度为41 mg/L。This study uses acetone extracting and high performance Liquid Chromatography to separate and purify biosurfaetant with prominent properties from bacteria fermentation fluid of thermophilie bacteria GeobaciUus thermoleovorans that take bioetyl as carbon resource. The biosurfaetant was identified as fatty acid glyeeride by the technique of methylation and acetylation derivation mixing with GC-MS/MS（ESI）. It could reduce surface tension of water to 32.7 mN · m^-1, and its critical mieelle concentration （CMC） was 41 mg · L^-1 , which all show that this biosurfactant has great potential in pharmaeeuties, environmental protection, cosmetic, oil recovery and many other fields.

关 键 词：生物表面活性剂喜热噬油芽胞杆菌甘油脂脂肪酸 

分 类 号：Q939.124[生物学—微生物学]