枯草芽孢杆菌B9601-Y_2基因组文库的构建  

Construction of a Genomic Library of Bacillus subtilis B9601-Y_2

作　　者：苏春丽[1] 何月秋[2] 

机构地区：[1]云南农业大学农业生物多样性与病虫害控制教育部重点实验室,云南昆明650201 [2]云南农业大学农学与生物技术学院,云南昆明650201

出　　处：《云南农业大学学报》2007年第6期799-801,807,共4页Journal of Yunnan Agricultural University

基　　金：国家自然科学基金(30660007)

摘　　要：枯草芽孢杆菌B9601-Y2是一个具有防治植物病害和促进植物生长的专利菌株。本文采用Sau3A I部分酶切的DNA、粘粒载体pLARF-5和PackageneλDNA包装系统,构建了该菌株基因组文库。文库总量为11 000个克隆,插入片段平均长度为14.77 kb,按99.99%的概率计算,文库覆盖了基因组4.2次。这为该菌株防治病害和促进植物生长机理研究奠定了物质基础。Bacillus subtilis B9601 - Y2 is a patented bioagent controlling fungal diseases and promoting growth of plants. Its genomic DNA library was constructed by partial digestion with Sau3A Ⅰ and cosmid vector pLAFR-5 following the Packagene Lambda DNA Packaging System. The library contains 11 000 clones with an average of 14. 77 kb insert, covering 4.2 genomic equivalences under 99.99% probability, which provides with basis the research on mechanisms of controlling diseases and promoting growth for the bacterium.

关 键 词：枯草芽孢杆菌B9601-Y2 基因组文库 粘粒载体 

分 类 号：Q933[生物学—微生物学]