缺氧对大鼠软脑膜微循环白细胞流变特性的影响  

Effects of Hypoxia on Rheologic Properties of Leukocytes in Pia Mater Microcirculation of Rats

作　　者：张钢[1] 高钰琪[1] 刘福玉[1] 

机构地区：[1]中国人民解放军第三军医大学高原军事医学系病理生理学与高原生理学教研室,全军高原生理与高原病研究重点实验室,邮政编码重庆400038

出　　处：《微循环学杂志》2007年第4期20-22,共3页Chinese Journal of Microcirculation

摘　　要：目的:探讨急、慢性缺氧对大鼠软脑膜微循环白细胞流变性的影响。方法:低压氧舱模拟大鼠急、慢性缺氧模型,开放式颅骨窗、活体显微镜观察和图像处理系统分析其白细胞的流变性。结果:急性缺氧大鼠白细胞沿血管壁滚动个数明显增多(P<0.05),与血管壁发生粘附数目显著增加(P<0.05),白细胞-内皮细胞接触时间(TLECT)明显延长(P<0.05);慢性缺氧大鼠白细胞粘附数目减少和TLECT缩短比较明显。结论:急性缺氧使白细胞流变性显著改变,可能是导致缺氧血管内皮细胞损伤的重要机制;慢性缺氧可使白细胞流变性一定程度恢复,对研究习服适应可能有重要意义。Objective: To investigate the effects of acute hypoxia and chronic hypoxia on the rheologic properties of leukocytes in pia mater microcirculation of rats.Method: Hypobaric chamber was used to simulate high altitude condition to reproduce animal hypoxic model, opening cranial window method, intravital microscopy and image analysis system were used to determine the rheologic properties of leukocytes in vivo. Results: The number of adhesive leukocytes and rolling leukocytes in venules were significantly increased(P<0.05) in acute hypoxic condition, and total leukocyte endothelial contact time (TLECT) was evidently extended (P<0.05).During chronic hypoxic condition, the number of adhesive leukocytes decreased and TLECT shortened at a certain extent. Conclusion: Acute hypoxia had a strong impact on the rheology of leukocytes and this may be the important mechanism of endothelium damage revoke by acute hypoxia.Chronic hypoxia ameliorated the rheologic properties of leukocytes in rats and this may facilitate rat acclimatization.

关 键 词：细胞流变特性 软脑膜微循环 白细胞活化 高原脑水肿 大鼠 缺氧 脑微循环障碍 内皮细胞粘附 

分 类 号：R543.502[医药卫生—心血管疾病]