姜黄素对单侧输尿管梗阻大鼠模型干预的研究  

Study on curcumin treatment in unilateral ureteral obstruction rats

作　　者：赵爱青[1] 张晓明[1] 候恒[1] 李荣山[2] 马存根[1] 

机构地区：[1]山西大同大学医学院内科教研室,山西大同037008 [2]山西医科大学第二临床医学院肾内科,山西太原030001

出　　处：《中国现代医学杂志》2007年第20期2449-2452,共4页China Journal of Modern Medicine

基　　金：山西省自然科学基金资助项目(项目编号:20021120)

摘　　要：目的观察姜黄素对单侧输尿管梗阻(UUO)大鼠肾小管间质病变的影响,探索防治肾间质纤维化的新途径。方法采用UUO致肾间质纤维化大鼠模型。将大鼠随机分为假手术组、模型组、姜黄素组。术后第4周,处死各组大鼠,用HE、Masson染色评定肾小管间质纤维化程度;用免疫组化方法检测肾组织内转化生长因子β1(TGF-β1)、基质金属蛋白酶抑制剂-1(TIMP-1)的表达部位及蛋白表达水平。结果与假手术组相比,肾间质纤维组织相对面积显著扩大(P<0.01),肾组织内TGF-β1和TIMP-1蛋白质表达均显著上调(P<0.01)。姜黄素干预后,上述上调指标都被显著抑制(P<0.01)。结论姜黄素具有抗肾纤维化的作用,其机制可能与下调肾组织TGF-β1进而抑制TIMP-1的表达有关。[Objective] To investigate the effect of curcumin on renal interstitial fibrosis following unilateral ureteral obstruction （UUO） and to explore new prevention and treatment for renal interstitial fibrosis. [Methods] Unilateral ureteral obstruction （UUO） rat models were used. Male wistar rats were randomly divided into three groups with 8 rats each： sham-operated group （0.9% saline solution）, model group （dimethyl sulphoxide, DMSO）, and curcumin treatment group （cureumin 100 mg/kg.d）. All rats were killed 4 weeks after surgery. The degree of tubulointerstitial fibrosis was scored by HE and Masson staining, and the sites and levels of protein expression of TGF-β1 and TIMP-1 were detected by immunohistochemistry staining. [Results] Compared with sham-operated group, the relative area of interstitial fibrosis was significantly enlarged in the model group （P 〈0.01）. Compared with sham-operated group, the expression of TGF-β1 and TIMP-1 protein were significantly up-regulated in their kidney tissue （P 〈0.01）. After intervention with cureumin, the upregnlation of the above-mentioned parameters were all significantly inhibited （P 〈0.01）. [ Conclusions] The effect of curcumin on ameliorating renal interstitial fibrosis may be correlated with its inhibition on over-expression of TGF-β1 and TIMP-1 in kidney tissue.

关 键 词：TGF—β 1 TIMP-1 姜黄素 肾间质纤维化 

分 类 号：R965[医药卫生—药理学]