两种方法检测肺癌患者CAP43蛋白的结果比较  

Immunohistochenmical examination and quatitative determination of Cap43 in lung cancer tissues

作　　者：何萍[1] 张志强[1] 戴晓淳[1] 傅立业[1] 隋承光[1] 孟凡东[1] 姜又红[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所第二研究室,辽宁沈阳110001

出　　处：《中国现代医学杂志》2007年第20期2477-2480,2484,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金(30471474)

摘　　要：目的探讨肺癌患者血清和肿瘤组织中Cap43蛋白的表达水平与肺癌不同组织学分型间的关系。方法通过建立双抗体夹心ELISA法初步定性和定量检测91肺癌患者与正常人群血清中Cap43蛋白含量,并采用免疫组化S-P方法检测83例肺癌术后病例的肿瘤组织切片中Cap43蛋白表达程度。结果初步建立的双抗ELISA血清学检测方法中确定的包被羊抗人NDRG1多克隆抗体,Ⅰ抗兔抗人Cap43多克隆抗体,辣根酶(HRP)标记的羊抗兔Ⅱ抗的最佳工作浓度分别为1∶750,1∶1200,1∶350,标准蛋白曲线的回归方程为:y=0.53566+0.00046x,R2=0.9939,P<0.0001。应用该方法初步检测结果提示肺癌患者血清腺癌组Cap43含量明显高于鳞癌组(P<0.05),同时免疫组化检测结果也提示肺腺癌组的Cap43表达程度比鳞癌组高(P<0.05)。结论肺癌患者血清中以及肿瘤组织Cap43的表达程度与其肿瘤的组织学类型密切相关。[Objective] To study the correlation between the intensity of Cap43expression in serum and tissues and tumor histological pattern in lung cancer. [Methods] 91 specimens were detected by double antibody sandwich ELISA for quantitatively detecting Cap43 protein in both lung cancer patients serum and non-lung cancer patients serum. 83 specimens were immunohistoehemieally stained using an antibody （NDRG1 antibody） against antiCap43 antigen in patient s with lung cancer. [Results] The optimal concentration of coating goat anti-human Cap43 PcAb, rabbit anti-human Cap43 and HRP-goat anti-rabbit Cap43 PcAb were 1：750, 1：1200, 1：350. A double antibody sandwich ELISA was developed for quantitatively detecting Cap43 pro in serum. Its linear detection was 10 to 3000 ng/mL （R2=0.9939, P 〈0.0001）. The intial quantitatibve detection showed the concentration of Cap43 pro in adenocarcinoma group was higher than squamous control group （P 〈0.05）. The S-Pimmunoehemieal also suggested that the Cap43 expression in the patient groups of adenoeareinoma increased significantly compared with the groups of squamous cell carcinoma （P 〈0.05）. [Conclusions] It is suggested that Cap43 expression in both serum and tissues is closely correlated with the tumor histological pattern in Lung cancer.

关 键 词：肺癌 CAP43 双抗体夹心ELISA 免疫组化S-P法 定量 图像分析 

分 类 号：R392.7[医药卫生—免疫学]