含绿色荧光蛋白基因的鸭肠炎病毒SD-01株US2基因缺失转移载体的构建  

Construction of US2 gene-deleted DEV SD-01 straintransfer vector containing EGFP

作　　者：李子剑[1] 李云龙[1] 陈苏[1] 李玉峰[2] 

机构地区：[1]山东师范大学生命科学学院,济南250023 [2]山东省农业科学院家禽研究所禽病研究中心

出　　处：《家禽科学》2007年第11期14-16,共3页Poultry Science

摘　　要：以鸭肠炎病毒(DEV)SD-01株DNA为模板,根据Gen Bank上已发表的基因序列设计一对引物,利用PCR技术扩增出US2全基因,将PCR产物连入pGM-T载体。用BamHI和EcoR V双酶切重组载体,使US2基因缺失约130bp,将含双loxP酶切位点的EGFP完整表达盒插入BamH I和EcoR V酶切位点之间,成功构建了以US2基因为重组臂的含EGFP基因的转移质粒载体pUS2-loxP-EGFP-loxP。DEV SD-01 strain genomic DNA was extracted and a pair of primers was synthesized according to the published nueleotide sequence in GenBank. The US2 gene was amplified by polymerase chain reaction （PCR） with extracted total DNA from DEV SD-01 strain as template and was cloned into vector pGM-T. The EGFP expression cassette flanked by two loxP sites was inserted between BamH I and EcoR V sites of the US2 gene to give rise to the transferring vector pUS2-loxP-EGFP-loxP.

关 键 词：鸭肠炎病毒 US2基因 EGFP基因 转移载体 

分 类 号：S852.65[农业科学—基础兽医学]