一种赤子爱胜蚓脱氧核糖核酸酶作用机制的研究  

Research of mechanism of action of a novel deoxyribonuclease from Eisenia foetida Eisenia foetida

作　　者：康慧芳[1] 刘志贞[1] 樊慧杰[1] 张建林[1] 罗佳[2] 杨利军[3] 牛勃[1] 

机构地区：[1]山西医科大学生物化学与分子生物学教研室,太原030001 [2]首都儿科研究所生物技术室 [3]山西医科大学科技处,太原030001

出　　处：《中国药物与临床》2007年第11期815-817,共3页Chinese Remedies & Clinics

基　　金：国家自然科学基金资助项目(30472251);山西省青年科技研究基金资助项目(20051044)

摘　　要：目的研究赤子爱胜蚓组织中脱氧核糖核酸酶1(EWD1)作用于底物的机制。方法采用ZORBAX-Oligo柱-高效液相色谱法(HPLC)、琼脂糖电泳、紫外分光光度法等分析技术,进行了蚯蚓EWD1对环状、线状DNA、单链DNA的降解中间产物和终产物的观察和分析。结果EWD1对所有形式的DNA底物均有降解作用,对单链DNA的降解产物为较均一的、<18bp的寡核苷酸,并显示EWD1无降解RNA的作用。结论蚯蚓组织中发现的EWD1能够分解多种来源的遗传物质,属于脱氧核糖核酸内切酶。Objective To study the mechanism underlying action of a novel deoxyribonuclease （EWD1） from Eisenia foetida. Methods ZORBAX-Oligo column HPLC, agarose electrophoresis and UV spectrophotometer analysis were used to investigate the intermediate and end products of EWD1 processed linear DNA, cyclic DNA and single strand DNA, and also the action of EWD1 on RNA substrates. Results EWD1 was shown to degrade all studied forms of DNA, yielding fairly size-consistent oligo-nucleotides as end-products （〈18 bp）. RNA substrates were not degradable with EWD1. Conclusion The EWD1 found in earthworm tissue was found to degrade genetic substance from various sources, although the underlying mechanism needs further exploration. The results indicated EWD1 to be of endonuclease nature （DNases）.

关 键 词：地龙 脱氧核糖核酸酶类 底物 

分 类 号：Q55[生物学—生物化学]