RP-HPLC法测定人血浆中莫西沙星的浓度  

Determination of Moxifloxacin in Human Serum by RP-HPLC

作　　者：贺德辉[1] 余江平[1] 

机构地区：[1]四川绵阳市中心医院,绵阳市621000

出　　处：《中国药房》2007年第32期2506-2507,共2页China Pharmacy

摘　　要：目的：建立以反相高效液相色谱法测定人血浆中莫西沙星浓度的方法。方法：色谱柱为GeminiC18，固定进样环为20μL，流动相为甲醇-水-三乙胺（112．5：208．9：0．06），流速为1mL·min^-1,紫外检测波长为289nm，柱温为25℃，灵敏度为0．01AUFS。结果：莫西沙星检测浓度在0．3125～10μg·mL^-1范围内线性关系良好（r=0．9998）；其低、中、高3种浓度（10、2．5、0．625μg·mL^-1）的回收率分别为99．46％、103．12％、96．14％；日内RSD分别为3．27％、3．35％、0．92％，日间RSD分别为7、19％、6．25％、6、68％。结论：本方法简单、快速、准确、灵敏度高，可用于莫西沙星的血药浓度测定及药动学研究。OBJECTIVE： To establish a RP- HPLC method for the determination of the concentration of moxifloxacin in human serum. METHODS：The separation of sample was performed on Gemini C18 column with a fixed sample injection volume of 20μL.The mobile phase was methanol- water- triethylamine（ll2.5 ： 208.9 ： 0.06） at a flow rate of 1mL · min^- 1.The UV detective wavelength was set at 289nm;the column temperature was 25℃ and the sensitivity was 0.01AUFS. RESULTS：In the range of 0.312 5--10μg · mL^-1（ r = 0.999 8）, moxifloxacin showed a good linear relation in HPLC.The recoveries of moxifloxacin at three concentrations（ 10, 2.5, 0.625μg · mL^-1） were 99.46%, 103.12%, and 96.14%, respectively, with intraday RSD at 3.27 %, 3.35 %, 0.92 %, respectively and inter - day RSD at 7.19 %, 6.25 %, 6.68 %, respectively. CONCLUSION ： This method is simple,rapid,accurate and sensitive,and it can be used for the concentration determination and the pharmacokinetic study of moxifloxacin.

关 键 词：反相高效液相色谱 莫西沙星 血药浓度 

分 类 号：R969.1[医药卫生—药理学]