中国荷斯坦牛BoLA-DRB3.2基因座的PCR-RFLP多态性分析  

Polymorphism Analysis of BoLA-DRB3.2 Gene in Chinese Holstein Cattle by PCR-RFLP

作　　者：赵福斌[1] 李建斌[1] 王洪梅[1] 胡桂学[2] 李秋玲[1] 仲跻峰[1] 侯明海[1] 

机构地区：[1]山东省农业科学院奶牛研究中心,山东济南250100 [2]吉林农业大学动物科技学院,吉林长春130118

出　　处：《家畜生态学报》2007年第5期25-27,30,共4页Journal of Domestic Animal Ecology

基　　金：国家863计划项目(No.2006AA10Z1D9);山东省农业科学院青年科研基金项目(2005YQ048);山东省农业良种工程项目(2003-2006)

摘　　要：主要组织复合体（Major Histocompatibility Complex，MHC）基因在动物机体免疫系统中具有重要作用，并与多种疾病的抗性或易感性存在相关性。利用限制性内切酶HaeⅢ和RsaⅠ通过FCR-RFLP技术分析了112头中国荷斯坦牛BoLA-DRB3．2基因的多态性。结果表明：所检测牛群中BoLA—DRB3．2基因在两个酶切位点均存在多态性，HaeⅢ酶切位点存在4种等位基因，有4种基因型；RsaⅠ酶切位点存在3种等位基因，有4种基因型。经χ^2适合性检验，所检测牛群中BoLA-DRB3．2基因在两个酶切位点均未达到Hardy-weinberg平衡状态。MHC（Major Histocompatibility Complex） plays a central role in immune system and associated with affectability or resistance of many diseases. The polymorphism of BoLA-DRB3. 2 in Chinese Holstein cattle was analyzed by PCR-RFLP. The results revealed DRB3.2 gene had extensive po phism,4 alleles and 4 genotypes digested with enzyme HaeⅢ ; 3 alleles and 4 genotypes digested w ymor- thenzyme Rsa Ⅰ. The Genotypic frequency and gene frequency of DRB3.2 gene in China Holstein Cattle was calculated. Statistical results showed that polymorphic locus for HaeⅢ and Rsa Ⅰ of China Holstein DRB3. 2 locus didn＇t fit with Hardy-weinberg equilibrium.

关 键 词：中国荷斯坦牛 BoLA-DRB3.2 基因多态性 PCR—RFLP 

分 类 号：S811.5[农业科学—畜牧学]